WB
Sample loading: Approximately 3 μg of total protein from Arabidopsis and rice leaves. Electrophoresis: 10% SDS-PAGE. Membrane transfer: Nitrocellulose (NC) membrane, 10V overnight. Blocking: 5% non-fat milk powder, room temperature incubation for 1h. Primary antibody: 1:5000 dilution, room temperature incubation for 1h.| Product Name | Anti-PAL1/ Phenylalanine ammonia-lyase 1 |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Recombinant partprotein of Arabidopsis AT2G37040，P35510 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB:1:2500-1:5000 |
|
| Species Reactivity | Arabidopsis |
| Purification | Affinity purified |
| Gene Symbol | PAL1 |
|---|---|
| Target Description | PAL1, a phenylalanine ammonia-lyase，is a key enzyme of plant metabolism catalyzing the first reaction in the biosynthesis from L-phenylalanine of a wide variety of natural products based on the phenylpropane skeleton. Arabidopsis has four PALs: AT2G37040 (PAL1), AT3G53260 (PAL2), AT5G04230 (PAL3) and AT3G10340 (PAL4). |
| Alternative Names | Phenylalanine ammonia-lyase 1 |
| Molecular Weight(MW) | 78kDa |
WB
Sample loading: Approximately 3 μg of total protein from Arabidopsis and rice leaves. Electrophoresis: 10% SDS-PAGE. Membrane transfer: Nitrocellulose (NC) membrane, 10V overnight. Blocking: 5% non-fat milk powder, room temperature incubation for 1h. Primary antibody: 1:5000 dilution, room temperature incubation for 1h.| Application Notes | WB:1:2500-1:5000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | 50% glycerol，-20 °C |
