WB
Human brain tissue were subjected to SDS PAGE followed by western blot with SETD1B antibody at dilution of 1:800 incubated at room temperature for 1.5 hours.IHC
Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using SETD1B antibody at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).ICC/IF
Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using SETD1B antibody at dilution of 1:200 and Omnimabs® 488 Goat Anti-Rabbit IgG(H&L), CL594-phalloidin (red).IP
IP result of anti-SETD1B (IP:5ug; Detection:1:300) with MCF-7 cells lysate 4mg.| Product Name | SETD1B Polyclonal antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Peptide |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHCIPWB |
| WB:1:500-1:1000 IHC:1:20-1:200 ICC/IF:1:50-1:500 IP:0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
|
| Species Reactivity | HumanMouse |
| Concentration | 0.7mg/ml |
| Purification | Affinity purified |
| Gene Symbol | SETD1B |
|---|---|
| Gene Synonyms | KMT2G Set1B IDDSELD |
| Gene Full Name | SET domain containing 1B, histone lysine methyltransferase |
| Gene Summary | SET1B is a component of a histone methyltransferase complex that produces trimethylated histone H3 at Lys4 (Lee et al., 2007 [PubMed 17355966]).[supplied by OMIM, Mar 2008] |
| Alternative Names | EC:2.1.1.364 Histone-lysine N-methyltransferase SETD1B hSET1B KIAA1076 KMT2G |
| Molecular Weight(MW) | 250-260 kDa, 450 kDa |
| Cellular Localization | Chromosome,Cytoplasm,Nucleus. |
WB
Human brain tissue were subjected to SDS PAGE followed by western blot with SETD1B antibody at dilution of 1:800 incubated at room temperature for 1.5 hours.
IHC
Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using SETD1B antibody at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
ICC/IF
Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using SETD1B antibody at dilution of 1:200 and Omnimabs® 488 Goat Anti-Rabbit IgG(H&L), CL594-phalloidin (red).
IP
IP result of anti-SETD1B (IP:5ug; Detection:1:300) with MCF-7 cells lysate 4mg.| Application Notes | WB:1:500-1:1000 IHC:1:20-1:200 ICC/IF:1:50-1:500 IP:0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20°C. Stable for one year after shipment. |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
