WB
Western blot analysis of various lysates using MonoMethyl-Histone H3-K4 Rabbit mAb at1:20000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 60s.ICC/IF
Confocal imaging of HeLa cells using MonoMethyl-Histone H3-K4 Rabbit mAb (dilution 1:300) (Green). The cells were counterstained with NF-kB p65/RelA Rabbit mAb (dilution 1:100) (Red). DAPI was used for nuclear staining (blue). Objective: 60x.DB
Dot-blot analysis of all sorts of peptides using MonoMethyl-Histone H3-K4 antibody at 1:2000 dilution.| Product Name | MonoMethyl-Histone H3-K4 Rabbit mAb |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | A synthetic monomethylated peptide around K4 of human Histone H3 (NP_003520.1). |
| Modification | MonoMethyl K4 |
| Clonality | monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | DBICC/IFWB |
| WB:1:2000-1:20000 ICC/IF:1:100-1:500 DB:1:1000-1:5000 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | H3C1 |
|---|---|
| Gene Synonyms | H3/A H3C2 H3C3 H3C4 H3C6 H3C7 H3C8 H3FA H3C10 H3C11 H3C12 HIST1H3A |
| Gene Full Name | H3 clustered histone 1 |
| Gene Summary | Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. This structure consists of approximately 146 bp of DNA wrapped around a nucleosome, an octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a replication-dependent histone that is a member of the histone H3 family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. This gene is found in the large histone gene cluster on chromosome 6p22-p21.3. [provided by RefSeq, Aug 2015] |
| Alternative Names | H3t H3.4 H3/g H3FT H3C16 HIST3H3 MonoMethyl-Histone H3-K4 |
| Molecular Weight(MW) | 17kDa |
| Cellular Localization | Chromosome, Nucleus. |
WB
Western blot analysis of various lysates using MonoMethyl-Histone H3-K4 Rabbit mAb at1:20000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 60s.
ICC/IF
Confocal imaging of HeLa cells using MonoMethyl-Histone H3-K4 Rabbit mAb (dilution 1:300) (Green). The cells were counterstained with NF-kB p65/RelA Rabbit mAb (dilution 1:100) (Red). DAPI was used for nuclear staining (blue). Objective: 60x.
DB
Dot-blot analysis of all sorts of peptides using MonoMethyl-Histone H3-K4 antibody at 1:2000 dilution.| Application Notes | WB:1:2000-1:20000 ICC/IF:1:100-1:500 DB:1:1000-1:5000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20℃. Avoid freeze / thaw cycles. |
| Storage Buffer | Buffer: PBS with 0.05% proclin300, 0.05% BSA, 50% glycerol, pH7.3. |
