WB
Western blot analysis of NK1.1 on NK1.1 recombinant protein with Rabbit anti-NK1.1 antibody at 1/1,000 dilution. Lysates/proteins at 50 ng/Lane. Exposure time: 11 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.IHC
Immunohistochemical analysis of paraffin-embedded C57BL/6 mouse thymus tissue with Rabbit anti-NK1.1 antibody at 1/2,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.| Product Name | NK1.1 Recombinant Rabbit Monoclonal Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Recombinant protein within mouse NK1.1 aa 67-223. |
| Clonality | monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IHCWB |
| WB:1:1000 IHC:1:2000 |
|
| Species Reactivity | Mouse |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Symbol | Klrb1c |
|---|---|
| Gene Synonyms | Nk1 Ly59 Nk-1 CD161 Ly-59 Ly55c NK1.1 NKRP1 Nk1.2 Klrb1b NK-RP1 Nk-1.2 Nkrp1b Nkrp1c ly-55c NKRP140 NKR-P1.9 |
| Gene Full Name | killer cell lectin-like receptor subfamily B member 1C |
| Gene Summary | Enables identical protein binding activity and signaling receptor activity. Acts upstream of or within natural killer cell activation and positive regulation of natural killer cell mediated cytotoxicity. Located in external side of plasma membrane. Is expressed in thymus primordium. Orthologous to human KLRB1 (killer cell lectin like receptor B1). [provided by Alliance of Genome Resources, Apr 2025] |
| Alternative Names | CD161 antigen like family member C antibody CD161 antigen-like family member C antibody CD161c antibody Killer cell lectin-like receptor subfamily B member 1C antibody Klrb1c antibody Klrb1f antibody KLRBC_MOUSE antibody Ly-55c antibody Ly55c antibody Lymphocyte antigen 55c antibody CD161 antigen like family member C antibody CD161 antigen-like family member C antibody CD161c antibody Killer cell lectin-like receptor subfamily B member 1C antibody Klrb1c antibody Klrb1f antibody KLRBC_MOUSE antibody Ly-55c antibody Ly55c antibody Lymphocyte antigen 55c antibody Lymphocyte antigen 59 antibody Natural killer cell surface protein P1-40 antibody NK1.1 antibody NKR P1.9 antibody NKR P1C antibody NKR-P1 40 antibody NKR-P1.9 antibody NKR-P1C antibody NKRP1 antibody |
| Molecular Weight(MW) | 25kDa |
| Cellular Localization | Membrane. |
WB
Western blot analysis of NK1.1 on NK1.1 recombinant protein with Rabbit anti-NK1.1 antibody at 1/1,000 dilution. Lysates/proteins at 50 ng/Lane. Exposure time: 11 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.
IHC
Immunohistochemical analysis of paraffin-embedded C57BL/6 mouse thymus tissue with Rabbit anti-NK1.1 antibody at 1/2,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.| Application Notes | WB:1:1000 IHC:1:2000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
