WB
Western blot analysis of extracts from Myeloma cells, using MED12 Antibody. The lane on the left was treated with blocking peptide.ICC/IF
MED12 Antibody staining HepG2 cells by ICC/IF. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100,then blocked in 10% serum for 45 minutes at 25°C. Samples were then incubated with primary Ab and mouse anti-beta tubulin Ab for 1 hour at 37°C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) Ab(Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) Ab(Green) were used as the secondary antibody. The nuclear counter stain is DAPI(blue).IHC
MED12 Antibody at 1/100 staining Rat kidney tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4°C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.| Product Name | Rabbit polyclonal antibody to MED12 |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | A synthesized peptide derived from human MED12(Accession Q93074), corresponding to amino acid residues S671-T721. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHCWB |
| WB:1:500-1:2000 ICC/IF:1:100-1:500 IHC:1:50-1:200 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | MED12 |
|---|---|
| Gene Synonyms | Kto OKS FGS1 HDKR HOPA OPA1 OHDOX ARC240 CAGH45 MED12S TNRC11 TRAP230 |
| Gene Full Name | mediator complex subunit 12 |
| Gene Summary | The initiation of transcription is controlled in part by a large protein assembly known as the preinitiation complex. A component of this preinitiation complex is a 1.2 MDa protein aggregate called Mediator. This Mediator component binds with a CDK8 subcomplex which contains the protein encoded by this gene, mediator complex subunit 12 (MED12), along with MED13, CDK8 kinase, and cyclin C. The CDK8 subcomplex modulates Mediator-polymerase II interactions and thereby regulates transcription initiation and reinitation rates. The MED12 protein is essential for activating CDK8 kinase. Defects in this gene cause X-linked Opitz-Kaveggia syndrome, also known as FG syndrome, and Lujan-Fryns syndrome. [provided by RefSeq, Aug 2009] |
| Alternative Names | Activator recruited cofactor 240 kDa component Activator-recruited cofactor 240 kDa component ARC240 CAG repeat protein 45 CAGH45 HOPA KIAA0192 MED12 MED12_HUMAN Mediator complex subunit 12 Mediator of RNA polymerase II transcription subunit 12 OPA containing protein OPA-containing protein Thyroid hormone receptor associated protein complex 230 kDa component Thyroid hormone receptor-associated protein complex 230 kDa component TNRC11 Trap230 Trinucleotide repeat containing gene 11 protein Trinucleotide repeat-containing gene 11 protein |
| Molecular Weight(MW) | 243kDa |
| Cellular Localization | Nucleus. |
WB
Western blot analysis of extracts from Myeloma cells, using MED12 Antibody. The lane on the left was treated with blocking peptide.
ICC/IF
MED12 Antibody staining HepG2 cells by ICC/IF. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100,then blocked in 10% serum for 45 minutes at 25°C. Samples were then incubated with primary Ab and mouse anti-beta tubulin Ab for 1 hour at 37°C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) Ab(Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) Ab(Green) were used as the secondary antibody. The nuclear counter stain is DAPI(blue).
IHC
MED12 Antibody at 1/100 staining Rat kidney tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4°C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.| Application Notes | WB:1:500-1:2000 ICC/IF:1:100-1:500 IHC:1:50-1:200 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20 °C. Stable for 12 months from date of receipt. |
| Storage Buffer | Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
