WB
Western blot analysis of lysates from C6 cells using Phospho-SMAD1-S463/S465+SMAD5 S463/S465+SMAD9-S465/S467 Rabbit mAb at 1:1000 dilution. C6 cells were treated by TGF beta 3(10ng/ml) and MG132(2 μM) at 37℃ for 20 minutes. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L)at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.IHC
Immunohistochemistry analysis of paraffin embedded Human lung cancer tissue using Phospho-SMAD1-S463/S465+SMAD5 S463/S465+SMAD9-S465/S467 Rabbit mAb at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.ICC/IF
Confocal imaging of HeLa cells (treated with BMP2) and HeLa cells (untreated) using Phospho-SMAD1-S463/S465+SMAD5 S463/S465+SMAD9-S465/S467 Rabbit mAb (dilution 1:200) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (dilution 1:500) (Red). The cells were counterstained with α-tubulin Mouse mAb (dilution 1:400) followed by incubation with 488-conjugated Goat Anti-Mouse IgG (H+L) Ab (dilution 1:500) (Green). DAPI was used for nuclear staining (Blue). Objective: 100x.| Product Name | Phospho-SMAD1-S463/S465+SMAD5-S463/S465+SMAD9-S465/S467 Rabbit mAb |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | A synthetic phosphorylated peptide around S463/S465//S467 of human SMAD1/SMAD5/SMAD9. |
| Modification | p-SMAD1-S463/S465+SMAD5-S463/S465+SMAD9-S465/S467 |
| Clonality | monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHCWB |
| WB:1:1000-1:2000 IHC:1:50-1:200 ICC/IF:1:100-1:400 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | BSP1 MADH1 MADR1 JV4-1 DWFC JV5-1 MADH5 MADH6 MADH9 SMAD8 Phospho-SMAD1-S463/S465+SMAD5-S463/S465+SMAD9-S465/S467 |
|---|---|
| Molecular Weight(MW) | Calculated MW 52kDa(Observed MW 60kDa) |
| Function | The protein encoded by this gene is involved in the transforming growth factor beta signaling pathway that results in an inhibition of the proliferation of hematopoietic progenitor cells. The encoded protein is activated by bone morphogenetic proteins type 1 receptor kinase, and may be involved in cancer. Alternative splicing results in multiple transcript variants. |
| Cellular Localization | Cytoplasm, Nucleus. |
WB
Western blot analysis of lysates from C6 cells using Phospho-SMAD1-S463/S465+SMAD5 S463/S465+SMAD9-S465/S467 Rabbit mAb at 1:1000 dilution. C6 cells were treated by TGF beta 3(10ng/ml) and MG132(2 μM) at 37℃ for 20 minutes. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L)at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.
IHC
Immunohistochemistry analysis of paraffin embedded Human lung cancer tissue using Phospho-SMAD1-S463/S465+SMAD5 S463/S465+SMAD9-S465/S467 Rabbit mAb at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.
ICC/IF
Confocal imaging of HeLa cells (treated with BMP2) and HeLa cells (untreated) using Phospho-SMAD1-S463/S465+SMAD5 S463/S465+SMAD9-S465/S467 Rabbit mAb (dilution 1:200) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (dilution 1:500) (Red). The cells were counterstained with α-tubulin Mouse mAb (dilution 1:400) followed by incubation with 488-conjugated Goat Anti-Mouse IgG (H+L) Ab (dilution 1:500) (Green). DAPI was used for nuclear staining (Blue). Objective: 100x.| Application Notes | WB:1:1000-1:2000 IHC:1:50-1:200 ICC/IF:1:100-1:400 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20℃. Avoid freeze / thaw cycles. |
| Storage Buffer | Buffer: PBS with 0.05% proclin300, 0.05% BSA, 50% glycerol, pH7.3. |
