WB
Western blot analysis of extracts from Mouse Cerebellum, using GODZ Antibody. The lane on the left was treated with blocking peptide.ICC/IF
GODZ Antibody staining Hela cells by ICC/IF. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100,then blocked in 10% serum for 45 minutes at 25°C. Samples were then incubated with primary Ab and mouse anti-beta tubulin Ab for 1 hour at 37°C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) Ab(Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) Ab(Green) were used as the secondary antibody. The nuclear counter stain is DAPI (blue).| Product Name | Rabbit polyclonal antibody to GODZ |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | A synthesized peptide derived from human ZDHHC3(Accession Q9NYG2), corresponding to amino acid residues K254-V299. |
| Clonality | polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFWB |
| WB:1:500-1:2000 ICC/IF:1:100-1:500 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | ZDHHC3 |
|---|---|
| Gene Synonyms | GODZ DHHC3 DHHC-3 ZNF373 |
| Gene Full Name | zDHHC palmitoyltransferase 3 |
| Gene Summary | Enables palmitoyltransferase activity. Involved in several processes, including protein localization to membrane; protein localization to photoreceptor outer segment; and regulation of signal transduction. Located in membrane. Is active in Golgi apparatus. [provided by Alliance of Genome Resources, Apr 2025] |
| Molecular Weight(MW) | 34kDa |
| Cellular Localization | Golgi apparatus membrane>Multi-pass membrane protein. |
WB
Western blot analysis of extracts from Mouse Cerebellum, using GODZ Antibody. The lane on the left was treated with blocking peptide.
ICC/IF
GODZ Antibody staining Hela cells by ICC/IF. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100,then blocked in 10% serum for 45 minutes at 25°C. Samples were then incubated with primary Ab and mouse anti-beta tubulin Ab for 1 hour at 37°C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) Ab(Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) Ab(Green) were used as the secondary antibody. The nuclear counter stain is DAPI (blue).| Application Notes | WB:1:500-1:2000 ICC/IF:1:100-1:500 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20 °C. Stable for 12 months from date of receipt. |
| Storage Buffer | Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
Data sheet for OM644252