WB
Various whole cell lysates were separated by 4-20% SDS-PAGE, and the membrane was blotted with anti-MYL9 (Phospho Thr18/Ser19) antibody. The HRP-conjugated Goat anti-Rabbit IgG (H + L) antibody was used to detect the antibody. Lane 1: Hela Lane, 2: Hela was treated with ML-7(75uM) for 30 minutes.IHC
Mouse cardiac muscle was stained with anti-MYL9 (Phospho Thr18/Ser19) rabbit antibody.| Product Name | MYL9 (Phospho Thr18/Ser19) Rabbit mAb |
|---|---|
| Antibody Type | Primary Antibodies |
| Modification | p-Thr18/Ser19 |
| Clonality | monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IHCWB |
| WB:1:1000-1:5000 IHC:1:50-1:400 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Symbol | MYL9 |
|---|---|
| Gene Synonyms | LC20 MLC2 MRLC1 MYRL2 MLC-2C MMIHS4 |
| Gene Full Name | myosin light chain 9 |
| Target Description | MYL9 (Phospho Thr18/Ser19) Antibody detects endogenous levels of MYL9 protein only when phosphorylated at Thr18/Ser19.The name of modified sites may be influenced by many factors, such as species (the modified site was not originally found in human samples) and the change of protein sequence (the previous protein sequence is incomplete, and the protein sequence may be prolonged with the development of protein sequencing technology). When naming, we will use the "numbers" in historical reference to keep the sites consistent with the reports. The antibody binds to the following modification sequence (lowercase letters are modification sites):RAtsNV |
| Molecular Weight(MW) | 20kDa |
| Cellular Localization | Cytoplasm, cytoskeleton . Cytoplasm, cell cortex . Colocalizes with F-actin, MYH9 and PIEZO1 at the actomyosin cortex in myoblasts. . |
WB
Various whole cell lysates were separated by 4-20% SDS-PAGE, and the membrane was blotted with anti-MYL9 (Phospho Thr18/Ser19) antibody. The HRP-conjugated Goat anti-Rabbit IgG (H + L) antibody was used to detect the antibody. Lane 1: Hela Lane, 2: Hela was treated with ML-7(75uM) for 30 minutes.
IHC
Mouse cardiac muscle was stained with anti-MYL9 (Phospho Thr18/Ser19) rabbit antibody.| Application Notes | WB:1:1000-1:5000 IHC:1:50-1:400 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | -15°C to -25°C/1 year(Do not lower than -25°C) |
| Storage Buffer | PBS, 50% glycerol, 0.05% Proclin 300, 0.05%BSA |
Data sheet for OM644253