WB
Western blot analysis of lysates from HeLa cells, using Phospho-Myosin Light Chain 2-S19 Rabbit mAb at 1:1000 dilution. HeLa cells were treated with Calyculin A (100 nM) at 37℃ for 30 minutesafter serum-starvation overnight.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 10s.WB
Western blot analysis of lysates from C2C12 cells, using Phospho-Myosin Light Chain 2-S19 Rabbit mAb at 1:1000 dilution. C2C12 cells were treated with Calyculin A (100 nM) at 37℃ for 30 minutesafter serum-starvation overnight.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 15s.| Product Name | Phospho-Myosin Light Chain 2-S19 Rabbit mAb |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Synthetic peptide. This information is considered to be commercially sensitive. |
| Modification | P-S19 |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB:1:1000-1:6000 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | MYL9 |
|---|---|
| Gene Synonyms | LC20 MLC2 MRLC1 MYRL2 MLC-2C MMIHS4 |
| Gene Full Name | myosin light chain 9 |
| Gene Summary | Myosin, a structural component of muscle, consists of two heavy chains and four light chains. The protein encoded by this gene is a myosin light chain that may regulate muscle contraction by modulating the ATPase activity of myosin heads. The encoded protein binds calcium and is activated by myosin light chain kinase. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008] |
| Alternative Names | LC20 MLC2 MRLC1 MYRL2 MLC-2C MMIHS4 Phospho-Myosin Light Chain 2-S19 |
| Molecular Weight(MW) | 20kDa(Observed band size:20kDa) |
| Cellular Localization | cell cortex, cytoplasm, cytosol, myofibril, Z disc. |
WB
Western blot analysis of lysates from HeLa cells, using Phospho-Myosin Light Chain 2-S19 Rabbit mAb at 1:1000 dilution. HeLa cells were treated with Calyculin A (100 nM) at 37℃ for 30 minutesafter serum-starvation overnight.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 10s.
WB
Western blot analysis of lysates from C2C12 cells, using Phospho-Myosin Light Chain 2-S19 Rabbit mAb at 1:1000 dilution. C2C12 cells were treated with Calyculin A (100 nM) at 37℃ for 30 minutesafter serum-starvation overnight.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 15s.| Application Notes | WB:1:1000-1:6000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20℃. Avoid freeze / thaw cycles. |
| Storage Buffer | PBS containing 50% glycerol and 0.05% BSA, preserved with proclin300 or sodium azide (as specified on the Certificate of Analysis), pH 7.3. |
