WB
HL-60 cells were subjected to SDS PAGE followed by western blot with DCT antibody at dilution of 1:1000 incubated at room temperature for 1.5 hours.IHC
Immunohistochemical analysis of paraffin-embedded human skin using DCT antibody at dilution of 1:100 (under 40x lens).ICC/IF
Immunofluorescent analysis of (-20°C Methanol) fixed A549 cells using DCT antibody at dilution of 1:400 and Omnimabs® 488 Goat Anti-Rabbit IgG(H&L).IP
IP result of anti-DCT (IP:4ug; Detection: 1:500) with HL-60 cells lysate 4mg.| Product Name | DCT Polyclonal antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Recombinant human DCT protein. |
| Clonality | polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHCIPWB |
| WB:1:500-1:1000 IHC:1:20-1:200 ICC/IF:1:200-1:800 IP:0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
|
| Species Reactivity | HumanMonkeyMouseRat |
| Concentration | 0.35mg/ml |
| Purification | Affinity purified |
| Gene Symbol | DCT |
|---|---|
| Gene Synonyms | OCA8 TRP-2 TYRP2 |
| Gene Full Name | dopachrome tautomerase |
| Gene Summary | Predicted to enable dopachrome isomerase activity. Involved in response to blue light. Located in intracellular membrane-bounded organelle and plasma membrane. Implicated in oculocutaneous albinism. [provided by Alliance of Genome Resources, Apr 2025] |
| Molecular Weight(MW) | 59kDa |
| Cellular Localization | Melanosome membrane,Melanosome. |
WB
HL-60 cells were subjected to SDS PAGE followed by western blot with DCT antibody at dilution of 1:1000 incubated at room temperature for 1.5 hours.
IHC
Immunohistochemical analysis of paraffin-embedded human skin using DCT antibody at dilution of 1:100 (under 40x lens).
ICC/IF
Immunofluorescent analysis of (-20°C Methanol) fixed A549 cells using DCT antibody at dilution of 1:400 and Omnimabs® 488 Goat Anti-Rabbit IgG(H&L).
IP
IP result of anti-DCT (IP:4ug; Detection: 1:500) with HL-60 cells lysate 4mg.| Application Notes | WB:1:500-1:1000 IHC:1:20-1:200 ICC/IF:1:200-1:800 IP:0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20°C. Stable for one year after shipment. |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Data sheet for OM644258