WB
Western blot analysis of extracts from various samples, using eNOS Mouse Monoclonal Antibody. Lane 1: Hepg2 cells, blocked with antigen-specific peptides. Lane 2: Hepg2 cells. Lane 3: A549 cells. Lane 4: Huvec cells. Lane 5: Hela cells.WB
Western blot analysis of extracts from various samples, using eNOS Mouse Monoclonal Antibody. Lane 1: Rat lung, blocked with antigen-specific peptides. Lane 2: Rat lung. Lane 3: Rat liver. Lane 4: Mouse liver. Lane 5: Mouse lung.IHC
eNOS Mouse Monoclonal Antibody at 1/100 staining rat brain tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4°C overnight. An HRP conjugated anti-mouse antibody was used as the secondary antibody.| Product Name | Mouse monoclonal antibody to eNOS |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | A synthesized peptide derived from human NOS3(Accession P29474), corresponding to amino acid residues L469-K519. |
| Clonality | monoclonal |
|---|---|
| Isotype | IgG1 |
| Host Species | Mouse |
| Tested Applications | IHCWB |
| WB:1:500-1:3000 IHC:1:50-1:200 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | NOS3 |
|---|---|
| Gene Synonyms | eNOS ECNOS |
| Gene Full Name | nitric oxide synthase 3 |
| Gene Summary | Nitric oxide is a reactive free radical which acts as a biologic mediator in several processes, including neurotransmission and antimicrobial and antitumoral activities. Nitric oxide is synthesized from L-arginine by nitric oxide synthases. Variations in this gene are associated with susceptibility to coronary spasm. Alternative splicing and the use of alternative promoters results in multiple transcript variants. [provided by RefSeq, Oct 2016] |
| Molecular Weight(MW) | 133kDa |
| Cellular Localization | Cell membrane. Membrane>Caveola. Cytoplasm>Cytoskeleton. Golgi apparatus. |
WB
Western blot analysis of extracts from various samples, using eNOS Mouse Monoclonal Antibody. Lane 1: Hepg2 cells, blocked with antigen-specific peptides. Lane 2: Hepg2 cells. Lane 3: A549 cells. Lane 4: Huvec cells. Lane 5: Hela cells.
WB
Western blot analysis of extracts from various samples, using eNOS Mouse Monoclonal Antibody. Lane 1: Rat lung, blocked with antigen-specific peptides. Lane 2: Rat lung. Lane 3: Rat liver. Lane 4: Mouse liver. Lane 5: Mouse lung.
IHC
eNOS Mouse Monoclonal Antibody at 1/100 staining rat brain tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4°C overnight. An HRP conjugated anti-mouse antibody was used as the secondary antibody.| Application Notes | WB:1:500-1:3000 IHC:1:50-1:200 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20 °C. Stable for 12 months from date of receipt. |
| Storage Buffer | Mouse IgG1 in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
Data sheet for OM644276