WB
Western blot analysis of CaV2.3 / CACNA1E on different lysates with Rabbit anti-CaV2.3 / CACNA1E antibody at 1/5,000 dilution. Lane 1: Mouse brain tissue lysate (no heat), Lane 2: Mouse hippocampus tissue lysate (no heat), Lane 3: Mouse lung tissue lysate (negative) (no heat), Lane 4: Rat brain tissue lysate (no heat), Notice: no heat means the lysate is not boiled. Lysates/proteins at 30 µg/Lane. Exposure time: 46 seconds; 3-8% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/5,000 dilution was used in primary antibody dilution at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.IHC
Immunohistochemical analysis of paraffin-embedded mouse hippocampus tissue with Rabbit anti-CaV2.3 / CACNA1E antibody at 1/6,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/6,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.IHC
Immunohistochemical analysis of paraffin-embedded rat hippocampus tissue with Rabbit anti-CaV2.3 / CACNA1E antibody at 1/6,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/6,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.IF-F
Species: Mouse, Site: hippocampus, Antibody concentration: 1/500. Antigen retrieval: Not required.| Product Name | CaV2.3 / CACNA1E Recombinant Rabbit Monoclonal Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Recombinant protein within human CACNA1E aa 1,964-2,313. |
| Clonality | monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IF-FIHCWB |
| WB:1:5000 IHC:1:3000-1:6000 IF-F:1:500 |
|
| Species Reactivity | MouseRat |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Symbol | CACNA1E |
|---|---|
| Gene Synonyms | BII CACH6 DEE69 gm139 Cav2.3 EIEE69 CACNL1A6 |
| Gene Full Name | calcium voltage-gated channel subunit alpha1 E |
| Gene Summary | Voltage-dependent calcium channels are multisubunit complexes consisting of alpha-1, alpha-2, beta, and delta subunits in a 1:1:1:1 ratio. These channels mediate the entry of calcium ions into excitable cells, and are also involved in a variety of calcium-dependent processes, including muscle contraction, hormone or neurotransmitter release, gene expression, cell motility, cell division and cell death. This gene encodes the alpha-1E subunit of the R-type calcium channels, which belong to the 'high-voltage activated' group that maybe involved in the modulation of firing patterns of neurons important for information processing. Alternatively spliced transcript variants encoding different isoforms have been described for this gene. [provided by RefSeq, Apr 2011] |
| Alternative Names | alpha-1 polypeptide antibody BII antibody Brain calcium channel II antibody CAC 1E antibody CAC1E antibody CAC1E_HUMAN antibody CACH 6 antibody CACH6 antibody CACNA1E antibody Cacnl1 a6 antibody alpha-1 polypeptide antibody BII antibody Brain calcium channel II antibody CAC 1E antibody CAC1E antibody CAC1E_HUMAN antibody CACH 6 antibody CACH6 antibody CACNA1E antibody Cacnl1 a6 antibody CACNL1A6 antibody Calcium channel antibody Calcium channel L type alpha 1 polypeptide isoform 6 antibody calcium channel R type antibody Cav 2.3 antibody Cchra 1 antibody Cchra1 antibody isoform 6 antibody L type antibody Voltage dependent R type calcium channel subunit alpha 1E antibody Voltage gated calcium channel subunit alpha Cav2.3 antibody Voltage-dependent R-type calcium channel subunit alpha-1E antibody Voltage-gated calcium channel subunit alpha Cav2.3 antibody |
| Molecular Weight(MW) | 262kDa(Observed band size: 257kDa) |
| Cellular Localization | Membrane. |
WB
Western blot analysis of CaV2.3 / CACNA1E on different lysates with Rabbit anti-CaV2.3 / CACNA1E antibody at 1/5,000 dilution. Lane 1: Mouse brain tissue lysate (no heat), Lane 2: Mouse hippocampus tissue lysate (no heat), Lane 3: Mouse lung tissue lysate (negative) (no heat), Lane 4: Rat brain tissue lysate (no heat), Notice: no heat means the lysate is not boiled. Lysates/proteins at 30 µg/Lane. Exposure time: 46 seconds; 3-8% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/5,000 dilution was used in primary antibody dilution at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.
IHC
Immunohistochemical analysis of paraffin-embedded mouse hippocampus tissue with Rabbit anti-CaV2.3 / CACNA1E antibody at 1/6,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/6,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
IHC
Immunohistochemical analysis of paraffin-embedded rat hippocampus tissue with Rabbit anti-CaV2.3 / CACNA1E antibody at 1/6,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/6,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
IF-F
Species: Mouse, Site: hippocampus, Antibody concentration: 1/500. Antigen retrieval: Not required.| Application Notes | WB:1:5000 IHC:1:3000-1:6000 IF-F:1:500 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
