WB
human brain tissue were subjected to SDS PAGE followed by western blot with C9orf72 antibody at dilution of 1:1000 incubated at room temperature for 1.5 hours.IHC
Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using C9orf72 antibody at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).ICC/IF
Immunofluorescent analysis of (-20°C Ethanol) fixed SH-SY5Y cells using C9orf72 antibody at dilution of 1:400 andOmnimabs® 488 Goat Anti-Mouse IgG(H&L).IP
IP result of anti-C9orf72 (IP:4ug; Detection:1:1000) with C6 cells lysate 1320ug.| Product Name | C9orf72 Monoclonal antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Recombinant human C9orf72 protein |
| Clonality | monoclonal |
|---|---|
| Isotype | IgG2a |
| Host Species | Mouse |
| Tested Applications | ICC/IFIHCIPWB |
| WB:1:500-1:1000 IHC:1:500-1:2000 ICC/IF:1:200-1:800 IP:0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Symbol | C9orf72 |
|---|---|
| Gene Synonyms | ALSFTD DENND9 FTDALS DENNL72 FTDALS1 |
| Gene Full Name | C9orf72-SMCR8 complex subunit |
| Gene Summary | The protein encoded by this gene plays an important role in the regulation of endosomal trafficking, and has been shown to interact with Rab proteins that are involved in autophagy and endocytic transport. Expansion of a GGGGCC repeat from 2-22 copies to 700-1600 copies in the intronic sequence between alternate 5' exons in transcripts from this gene is associated with 9p-linked ALS (amyotrophic lateral sclerosis) and FTD (frontotemporal dementia) (PMID: 21944778, 21944779). Studies suggest that hexanucleotide expansions could result in the selective stabilization of repeat-containing pre-mRNA, and the accumulation of insoluble dipeptide repeat protein aggregates that could be pathogenic in FTD-ALS patients (PMID: 23393093). Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Jul 2016] |
| Alternative Names | 3D2H6 C9RANT DENND9 DENNL72 Guanine nucleotide exchange factor C9orf72 |
| Molecular Weight(MW) | 55kDa |
| Cellular Localization | Nucleus,Cell projection,Cytoplasm,Cytoplasmic vesicle,Endosome,Lysosome,Secreted. |
WB
human brain tissue were subjected to SDS PAGE followed by western blot with C9orf72 antibody at dilution of 1:1000 incubated at room temperature for 1.5 hours.
IHC
Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using C9orf72 antibody at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
ICC/IF
Immunofluorescent analysis of (-20°C Ethanol) fixed SH-SY5Y cells using C9orf72 antibody at dilution of 1:400 andOmnimabs® 488 Goat Anti-Mouse IgG(H&L).
IP
IP result of anti-C9orf72 (IP:4ug; Detection:1:1000) with C6 cells lysate 1320ug.| Application Notes | WB:1:500-1:1000 IHC:1:500-1:2000 ICC/IF:1:200-1:800 IP:0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20°C. Stable for one year after shipment. |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
