WB
Various whole cell lysates were separated by 4-20% SDS-PAGE, and the membrane was blotted with anti-C9orf72 antibody. The HRP-conjugated Goat anti-Rabbit IgG (H + L) antibody was used to detect the antibody. Lane 1: SH-SY5Y,Lane 2: Mouse brain ,Lane 2: C6 .IHC
Human cerebellum was stained with anti-C9orf72 Rabbit antibody.| Product Name | C9orf72 Rabbit mAb |
|---|---|
| Antibody Type | Primary Antibodies |
| Clonality | monoclonal |
|---|---|
| Isotype | IgG,K |
| Host Species | Rabbit |
| Tested Applications | IHCWB |
| WB:1:1000-1:20000 IHC:1:500-1:2000 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Symbol | C9orf72 |
|---|---|
| Gene Synonyms | ALSFTD DENND9 FTDALS DENNL72 FTDALS1 |
| Gene Full Name | C9orf72-SMCR8 complex subunit |
| Gene Summary | The protein encoded by this gene plays an important role in the regulation of endosomal trafficking, and has been shown to interact with Rab proteins that are involved in autophagy and endocytic transport. Expansion of a GGGGCC repeat from 2-22 copies to 700-1600 copies in the intronic sequence between alternate 5' exons in transcripts from this gene is associated with 9p-linked ALS (amyotrophic lateral sclerosis) and FTD (frontotemporal dementia) (PMID: 21944778, 21944779). Studies suggest that hexanucleotide expansions could result in the selective stabilization of repeat-containing pre-mRNA, and the accumulation of insoluble dipeptide repeat protein aggregates that could be pathogenic in FTD-ALS patients (PMID: 23393093). Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Jul 2016] |
| Molecular Weight(MW) | 54kDa(Observed MW 55kDa) |
| Cellular Localization | Nucleus,Cytoplasm. |
WB
Various whole cell lysates were separated by 4-20% SDS-PAGE, and the membrane was blotted with anti-C9orf72 antibody. The HRP-conjugated Goat anti-Rabbit IgG (H + L) antibody was used to detect the antibody. Lane 1: SH-SY5Y,Lane 2: Mouse brain ,Lane 2: C6 .
IHC
Human cerebellum was stained with anti-C9orf72 Rabbit antibody.| Application Notes | WB:1:1000-1:20000 IHC:1:500-1:2000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | -15°C to -25°C/1 year(Do not lower than -25°C) |
| Storage Buffer | PBS, 50% glycerol, 0.05% Proclin 300, 0.05%BSA |
