WB
Western blot analysis of histone H10 expression in Hep G2 (A), Sol8 (B), RAW 264.7 (C), RBL-1 (D), and L8 (E) whole cell lysates.IHC
Immunoperoxidase staining of human duodenal tissue fixed in formalin and embedded in paraffin reveals nuclear staining of glandular cells.ICC/IF
Immunofluorescence staining of A431 cells fixed in formalin reveals nuclear localization.| Product Name | Mouse monoclonal antibody to Histone H10 |
|---|---|
| Antibody Type | Primary Antibodies |
| Clonality | monoclonal |
|---|---|
| Isotype | IgG1, k |
| Host Species | Mouse |
| Tested Applications | ICC/IFIHCWB |
| WB:1:100-1:1000 IHC:1:50-1:500 ICC/IF:1:50-1:500 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 0.2mg/ml |
| Purification | Affinity purified |
| Gene Symbol | H1-0 |
|---|---|
| Gene Synonyms | H10 H1.0 H1F0 H1FV |
| Gene Full Name | H1.0 linker histone |
| Gene Summary | Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a replication-independent histone that is a member of the histone H1 family. [provided by RefSeq, Oct 2015] |
| Molecular Weight(MW) | 32kDa |
| Cellular Localization | Nucleus. |
WB
Western blot analysis of histone H10 expression in Hep G2 (A), Sol8 (B), RAW 264.7 (C), RBL-1 (D), and L8 (E) whole cell lysates.
IHC
Immunoperoxidase staining of human duodenal tissue fixed in formalin and embedded in paraffin reveals nuclear staining of glandular cells.
ICC/IF
Immunofluorescence staining of A431 cells fixed in formalin reveals nuclear localization.| Application Notes | WB:1:100-1:1000 IHC:1:50-1:500 ICC/IF:1:50-1:500 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4° C, **DO NOT FREEZE**. Stable for one year from the date of shipment. Non-hazardous. No MSDS required. |
| Storage Buffer | Each vial contains 200 µg IgG1 kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin. |
