WB
Western blot analysis of CRALBP on different lysates with Rabbit anti-CRALBP antibody at 1/1,000 dilution. Lane 1: SK-MEL-28 cell lysate, Lane 2: HepG2 cell lysate (negative cell), Lane 3: Mouse eyeball tissue lysate, Lane 4: Rat eyeball tissue lysate, Lysates/proteins at 30 µg/Lane. Exposure time: 43 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.IHC
Immunohistochemical analysis of paraffin-embedded mouse retina tissue with Rabbit anti-CRALBP antibody at 1/500 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.IF-F
Species: Mouse, Site: Retina, Sample: Frozen section, Antibody concentration: 1/500, Antigen retrieval: Not required.IF-P
Species: Mouse, Site: Retina, Sample: Paraffin-embedded section, Antibody concentration: 1/200.| Product Name | CRALBP Recombinant Rabbit Monoclonal Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Recombinant protein within human CRALBP aa 1-317 / 317. |
| Clonality | monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IF-FIF-PIHCWB |
| WB:1:1000-1:5000 IHC:1:500-1:1000 IF-F:1:500 IF-P:1:200 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Symbol | RLBP1 |
|---|---|
| Gene Synonyms | CRALBP |
| Gene Full Name | retinaldehyde binding protein 1 |
| Gene Summary | The protein encoded by this gene is a 36-kD water-soluble protein which carries 11-cis-retinaldehyde or 11-cis-retinal as physiologic ligands. It may be a functional component of the visual cycle. Mutations of this gene have been associated with severe rod-cone dystrophy, Bothnia dystrophy (nonsyndromic autosomal recessive retinitis pigmentosa) and retinitis punctata albescens. [provided by RefSeq, Jul 2008] |
| Alternative Names | Cellular retinaldehyde binding protein 1 antibody Cellular retinaldehyde binding protein antibody Cellular retinaldehyde-binding protein antibody MGC3663 antibody Retinaldehyde binding protein 1 antibody Retinaldehyde-binding protein 1 antibody RLBP 1 antibody RLBP1 antibody RLBP1_HUMAN antibody |
| Molecular Weight(MW) | 36kDa(Observed band size:30kDa) |
| Cellular Localization | Cytoplasm. |
WB
Western blot analysis of CRALBP on different lysates with Rabbit anti-CRALBP antibody at 1/1,000 dilution. Lane 1: SK-MEL-28 cell lysate, Lane 2: HepG2 cell lysate (negative cell), Lane 3: Mouse eyeball tissue lysate, Lane 4: Rat eyeball tissue lysate, Lysates/proteins at 30 µg/Lane. Exposure time: 43 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.
IHC
Immunohistochemical analysis of paraffin-embedded mouse retina tissue with Rabbit anti-CRALBP antibody at 1/500 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
IF-F
Species: Mouse, Site: Retina, Sample: Frozen section, Antibody concentration: 1/500, Antigen retrieval: Not required.
IF-P
Species: Mouse, Site: Retina, Sample: Paraffin-embedded section, Antibody concentration: 1/200.| Application Notes | WB:1:1000-1:5000 IHC:1:500-1:1000 IF-F:1:500 IF-P:1:200 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
