WB
Positive WB detected in: Rat spleen tissue, All lanes: IL2RA antibody at 3.2μg/ml, Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution.IHC
Immunohistochemistry of paraffin-embedded human glioma using IL2RA Antibody at dilution of 1:100.ICC/IF
Immunofluorescent analysis of HepG2 cells using IL2RA Antibody at dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).| Product Name | Rabbit anti-IL2RA Polyclonal antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Recombinant Human Interleukin-2 receptor subunit alpha protein (22-185AA). |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHCWB |
| WB:1:500-1:5000 IHC:1:20-1:200 ICC/IF:1:50-1:200 |
|
| Species Reactivity | HumanRat |
| Concentration | 1mg/ml |
| Purification | Protein G |
| Gene Symbol | IL2RA |
|---|---|
| Gene Synonyms | p55 CD25 IL2R IMD41 TCGFR IDDM10 |
| Gene Full Name | interleukin 2 receptor subunit alpha |
| Gene Summary | The interleukin 2 (IL2) receptor alpha (IL2RA) and beta (IL2RB) chains, together with the common gamma chain (IL2RG), constitute the high-affinity IL2 receptor. Homodimeric alpha chains (IL2RA) result in low-affinity receptor, while homodimeric beta (IL2RB) chains produce a medium-affinity receptor. Normally an integral-membrane protein, soluble IL2RA has been isolated and determined to result from extracellular proteolyisis. Alternately-spliced IL2RA mRNAs have been isolated, but the significance of each is presently unknown. Mutations in this gene are associated with interleukin 2 receptor alpha deficiency. Patients with severe Coronavirus Disease 2019 (COVID-19), the disease caused by the novel severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), have significantly elevated levels of IL2R in their plasma. Similarly, serum IL-2R levels are found to be elevated in patients with different types of carcinomas. Certain IL2RA and IL2RB gene polymorphisms have been associated with lung cancer risk. [provided by RefSeq, Jul 2020] |
| Alternative Names | IL2RA Interleukin-2 receptor subunit alpha IL-2 receptor subunit alpha IL-2-RA IL-2R subunit alpha IL2-RA TAC antigen p55 CD antigen CD25 |
| Molecular Weight(MW) | 31kDa |
| Cellular Localization | Membrane. |
WB
Positive WB detected in: Rat spleen tissue, All lanes: IL2RA antibody at 3.2μg/ml, Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution.
IHC
Immunohistochemistry of paraffin-embedded human glioma using IL2RA Antibody at dilution of 1:100.
ICC/IF
Immunofluorescent analysis of HepG2 cells using IL2RA Antibody at dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).| Application Notes | WB:1:500-1:5000 IHC:1:20-1:200 ICC/IF:1:50-1:200 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Upon receipt, store at -20°C or -80°C. Avoid repeated freeze. |
| Storage Buffer | Preservative: 0.03% Proclin 300,Constituents: 50% Glycerol, 0.01M PBS, pH 7.4 |
