WB
Various lysates were subjected to SDS PAGE followed by western blot with Cannabinoid receptor 1 antibody at dilution of 1:2000 incubated at room temperature for 1.5 hours.IHC
Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using Cannabinoid receptor 1 antibody at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).IF-P
Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using Cannabinoid receptor 1 antibody at dilution of 1:200 and Omnimabs® 488 Goat Anti-Rabbit IgG(H&L).| Product Name | Cannabinoid receptor 1 Polyclonal antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Recombinant human CNR1 protein. |
| Clonality | polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IF-PIHCWB |
| WB:1:1000-1:4000 IHC: 1:200-1:800 IF-P:1:50-1:500 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 0.4mg/ml |
| Purification | Affinity purified |
| Gene Symbol | CNR1 |
|---|---|
| Gene Synonyms | CB1 CNR CB-R CB1A CB1R CANN6 CB1K5 |
| Gene Full Name | cannabinoid receptor 1 |
| Gene Summary | This gene encodes one of two cannabinoid receptors. The cannabinoids, principally delta-9-tetrahydrocannabinol and synthetic analogs, are psychoactive ingredients of marijuana. The cannabinoid receptors are members of the guanine-nucleotide-binding protein (G-protein) coupled receptor family, which inhibit adenylate cyclase activity in a dose-dependent, stereoselective and pertussis toxin-sensitive manner. The two receptors have been found to be involved in the cannabinoid-induced CNS effects (including alterations in mood and cognition) experienced by users of marijuana. Multiple transcript variants encoding two different protein isoforms have been described for this gene. [provided by RefSeq, May 2009] |
| Molecular Weight(MW) | 53kDa(Observed MW:45/66kDa) |
| Cellular Localization | Cell membrane. |
WB
Various lysates were subjected to SDS PAGE followed by western blot with Cannabinoid receptor 1 antibody at dilution of 1:2000 incubated at room temperature for 1.5 hours.
IHC
Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using Cannabinoid receptor 1 antibody at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IF-P
Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using Cannabinoid receptor 1 antibody at dilution of 1:200 and Omnimabs® 488 Goat Anti-Rabbit IgG(H&L).| Application Notes | WB:1:1000-1:4000 IHC: 1:200-1:800 IF-P:1:50-1:500 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20°C. Stable for one year after shipment. |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Data sheet for OM644344