WB
Sample loading: Approximately 3 μg of total proteins from Arabidopsis thaliana and rice leaves. Electrophoresis: 12.5% SDS - PAGE. Membrane transfer: Nitrocellulose (NC) membrane, transfer at 10 V overnight. Blocking: Block with 5% non - fat dry milk at room temperature for 1 hour. Primary antibody: Dilute the primary antibody at a ratio of 1:5000 and incubate at room temperature for 1 hour.| Product Name | Anti-HSP70-6/ Heat shock 70 kDa protein 6, chloroplastic |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Recombinant part protein of Arabidopsis thaliana AT4G24280，Q9STW6 |
| Clonality | polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB:1:1000-1:5000 |
|
| Species Reactivity | ArabidopsisRice |
| Purification | Affinity purified |
| Gene Symbol | cpHsc70-1 |
|---|---|
| Gene Synonyms | chloroplast heat shock protein 70-1 T22A6.110 T22A6_110 |
| Gene Full Name | chloroplast heat shock protein 70-1 |
| Gene Summary | Involved in protein import into chloroplasts during early developmental stages. |
| Molecular Weight(MW) | 76.5 kDa for Arabidopsis thaliana |
WB
Sample loading: Approximately 3 μg of total proteins from Arabidopsis thaliana and rice leaves. Electrophoresis: 12.5% SDS - PAGE. Membrane transfer: Nitrocellulose (NC) membrane, transfer at 10 V overnight. Blocking: Block with 5% non - fat dry milk at room temperature for 1 hour. Primary antibody: Dilute the primary antibody at a ratio of 1:5000 and incubate at room temperature for 1 hour.| Application Notes | WB:1:1000-1:5000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | 50% glycerol，-20 °C |
Data sheet for OM644347