WB
Western blot analysis of MICAL3 using anti-MICAL3 antibody. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human U251 whole cell lysates, Lane 2: human SiHa whole cell lysates, Lane 3: human A549 whole cell lysates, Lane 4: human Hacat whole cell lysates, Lane 5: rat testis tissue lysates, Lane 6: mouse testis tissue lysates. After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-MICAL3 antigen affinity purified polyclonal antibody at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody. The signal is developed using ECL Plus Western Blotting Substrate.FC
Flow Cytometry analysis of U251 cells using anti-MICAL3 antibody. Overlay histogram showing U251 cells stained with anti-MICAL3 antibody (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-MICAL3 Antibody at 1:100 dilution for 30 min at 20°C. Omnimabs® 488 Goat Anti-Rabbit IgG(H&L) was used as secondary antibody at 1:100 dilution for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG at 1:100 dilution used under the same conditions. Unlabelled sample (Red line) was also used as a control.| Product Name | Rabbit polyclonal antibody to MICAL3 |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | E.coli-derived human MICAL3 recombinant protein (Position: K458-L1592). Human MICAL3 shares 81.9% amino acid (aa) sequence identity with mouse MICAL3. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | FCWB |
| WB:1:500-1:2000 FC:1:50-1:200 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 0.5mg/ml |
| Purification | Affinity purified |
| Gene Symbol | MICAL3 |
|---|---|
| Gene Synonyms | MICAL-3 |
| Gene Full Name | microtubule associated monooxygenase, calponin and LIM domain containing 3 |
| Gene Summary | Enables actin binding activity and molecular adaptor activity. Involved in actin filament depolymerization. Located in several cellular components, including Flemming body; intercellular bridge; and nucleoplasm. [provided by Alliance of Genome Resources, May 2025] |
| Alternative Names | MICAL3 KIAA0819 KIAA1364 [F-actin]-monooxygenase MICAL3 Molecule interacting with CasL protein 3 MICAL-3 |
| Molecular Weight(MW) | 224kDa(Observed MW 224-280kDa) |
| Cellular Localization | Cell projection,Cytoplasm,Cytoskeleton,Nucleus. |
WB
Western blot analysis of MICAL3 using anti-MICAL3 antibody. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human U251 whole cell lysates, Lane 2: human SiHa whole cell lysates, Lane 3: human A549 whole cell lysates, Lane 4: human Hacat whole cell lysates, Lane 5: rat testis tissue lysates, Lane 6: mouse testis tissue lysates. After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-MICAL3 antigen affinity purified polyclonal antibody at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody. The signal is developed using ECL Plus Western Blotting Substrate.
FC
Flow Cytometry analysis of U251 cells using anti-MICAL3 antibody. Overlay histogram showing U251 cells stained with anti-MICAL3 antibody (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-MICAL3 Antibody at 1:100 dilution for 30 min at 20°C. Omnimabs® 488 Goat Anti-Rabbit IgG(H&L) was used as secondary antibody at 1:100 dilution for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG at 1:100 dilution used under the same conditions. Unlabelled sample (Red line) was also used as a control.| Application Notes | WB:1:500-1:2000 FC:1:50-1:200 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | 12 months from date of receipt, -20℃ as supplied. 6 months 2 to 8℃ after reconstitution. Avoid repeated freezing and thawing. |
| Storage Buffer | 500ug/ml antibody with PBS, 0.02% NaN3, 1 mg/ml BSA and 50% glycerol. |
