WB
Western blot analysis of extracts of various cell lines, using RBCK1 an tibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection:ECL Basic Kit. Exposure time:90s.ICC/IF
Immunofluorescence analysis of NIH/3T3 cells using RBCK1 antibody at dilution of 1:100. Blue: DAPI for nuclear staining.| Product Name | RBCK1 polyclonal antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Recombinant fusion protein of human RBCK1(NP_112506.2). |
| Clonality | polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFWB |
| WB:1:500-1:2000 ICC/IF:1:50-1:200 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | RBCK1 |
|---|---|
| Gene Synonyms | XAP3 XAP4 HOIL1 PBMEI PGBM1 RBCK2 RNF54 HOIL-1 ZRANB4 C20orf18 UBCE7IP3 |
| Gene Full Name | RANBP2-type and C3HC4-type zinc finger containing 1 |
| Gene Summary | Enables several functions, including identical protein binding activity; transcription coactivator activity; and ubiquitin binding activity. Involved in several processes, including positive regulation of canonical NF-kappaB signal transduction; protein linear polyubiquitination; and regulation of DNA-binding transcription factor activity. Part of LUBAC complex. Implicated in glycogen storage disease. [provided by Alliance of Genome Resources, May 2025] |
| Molecular Weight(MW) | 58kDa |
| Cellular Localization | cytosol. |
WB
Western blot analysis of extracts of various cell lines, using RBCK1 an tibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection:ECL Basic Kit. Exposure time:90s.
ICC/IF
Immunofluorescence analysis of NIH/3T3 cells using RBCK1 antibody at dilution of 1:100. Blue: DAPI for nuclear staining.| Application Notes | WB:1:500-1:2000 ICC/IF:1:50-1:200 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles. |
| Storage Buffer | 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2. |
Data sheet for OM644364