ICC/IF
Immunofluorescence analysis of A549 cells using RBCK1 antibody (green). Blue: DAPI fluorescent DNA dye. Cells are fixed in 4% paraformaldehyde at room temperature for 20 minutes. Then, they are permeabilized with a PBS (OM750003) solution containing 0.1% Triton X-100(OM750021) at room temperature for 15 minutes. Subsequently, the cells are blocked with 10% non - immune goat serum(OM760028) at room temperature for 1 hour.The cells are incubated overnight at 4°C with the primary antibody diluted 1:100 in PBS. The secondary antibody, Omnimabs® 488 Goat Ant-Rabbit IgG(H&L) (Green,OM643486), is diluted at a ratio of 1:400 and incubated with the cells for 1 hour.Nuclear DNA is labeled with DAPI (Blue,OM643160).ELISA
Coat the plate with immunizing antigen.Blue line： Immune serum; Orange line: Pre-immune serum.| Product Name | Anti-RBCK1 antibody,pbs only |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Polypeptide |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAICC/IF |
| ICC/IF:1:100-1:500 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Symbol | RBCK1 |
|---|---|
| Gene Synonyms | C20orf18 HOIL-1 HOIL-1L HOIL1 PBMEI PGBM1 Pkcbpb15 RBCK2 RNF54 UBCE7IP3 UIP28 XAP3 XAP4 ZRANB4 |
| Gene Full Name | RANBP2-type and C3HC4-type zinc finger containing 1 |
| Gene Summary | The protein encoded by this gene is similar to mouse UIP28/UbcM4 interacting protein. Alternative splicing has been observed at this locus, resulting in distinct isoforms. |
| Alternative Names | HBV-associated factor 4 heme-oxidized IRP2 ubiquitin ligase 1 heme-oxidized IRP2 ubiquitin ligase 1 homolog hepatitis B virus X-associated protein 4 novel zinc finger C3HC4 type (RING finger) protein protein kinase C-binding protein beta-15 protein kinase C-binding protein Beta15 RanBP-type and C3HC4-type zinc finger containing 1 RBCC protein interacting with PKC 1 RBCC protein interacting with PKC1 RING finger protein 54 RING-type E3 ubiquitin transferase HOIL-1 ubcM4-interacting protein 28 ubiquitin conjugating enzyme 7 interacting protein 3 ubiquitin-conjugating enzyme 7-interacting protein 3 |
| Molecular Weight(MW) | 58 kDa |
| Cellular Localization | Cytosol |
| SwissProt ID | Q9BYM8 |
|---|
ICC/IF
Immunofluorescence analysis of A549 cells using RBCK1 antibody (green). Blue: DAPI fluorescent DNA dye. Cells are fixed in 4% paraformaldehyde at room temperature for 20 minutes. Then, they are permeabilized with a PBS (OM750003) solution containing 0.1% Triton X-100(OM750021) at room temperature for 15 minutes. Subsequently, the cells are blocked with 10% non - immune goat serum(OM760028) at room temperature for 1 hour.The cells are incubated overnight at 4°C with the primary antibody diluted 1:100 in PBS. The secondary antibody, Omnimabs® 488 Goat Ant-Rabbit IgG(H&L) (Green,OM643486), is diluted at a ratio of 1:400 and incubated with the cells for 1 hour.Nuclear DNA is labeled with DAPI (Blue,OM643160).
ELISA
Coat the plate with immunizing antigen.Blue line： Immune serum; Orange line: Pre-immune serum.| Application Notes | ICC/IF:1:100-1:500 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C long term. Avoid freeze / thaw cycle. |
| Storage Buffer | PBS ONLY |
