WB
Western blot analysis of extracts from various samples, using Integrin alpha 3 Antibody. Lane 1: A549 cells, blocked with antigen-specific peptides, Lane 2: A549 cells, Lane 3: Hela cells(H2O2 treatment).IHC
Integrin alpha 3 Antibody at 1/100 staining Human esophageal cancer and adjacent normal tissues by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4°C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.ICC/IF
Integrin alpha 3 Antibody staining Hela by ICC/IF. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100,then blocked in 10% serum for 45 minutes at 25°C. Samples were then incubated with primary Ab(1:200) and mouse anti-beta tubulin Ab(1:200) for 1 hour at 37°C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) Ab(Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) Ab(Green) were used as the secondary antibody. The nuclear counter stain is DAPI(blue).| Product Name | Rabbit polyclonal antibody to Integrin alpha 3 |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | A synthesized peptide derived from human ITGA3(Accession P26006), corresponding to amino acid residues C496-P546. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHCWB |
| WB:1:500-1:2000 IHC:1:50-1:200 ICC/IF:1:100-1:500 |
|
| Species Reactivity | HumanMonkeyMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | ITGA3 |
|---|---|
| Gene Synonyms | JEB7 VL3A CD49C FRP-2 GAPB3 ILNEB MSK18 VCA-2 VLA3a GAP-B3 |
| Gene Full Name | integrin subunit alpha 3 |
| Gene Summary | The gene encodes a member of the integrin alpha chain family of proteins. Integrins are heterodimeric integral membrane proteins composed of an alpha chain and a beta chain that function as cell surface adhesion molecules. The encoded preproprotein is proteolytically processed to generate light and heavy chains that comprise the alpha 3 subunit. This subunit joins with a beta 1 subunit to form an integrin that interacts with extracellular matrix proteins including members of the laminin family. Expression of this gene may be correlated with breast cancer metastasis. [provided by RefSeq, Oct 2015] |
| Alternative Names | AA407068 Antigen identified by monoclonal antibody J143 CD49 antigen like family member C CD49 antigen-like family member C CD49c CD49c antigen FLJ34631 FLJ34704 FRP 2 FRP-2 FRP2 Galactoprotein B3 GAP B3 GAPB3 Integrin alpha 3 Integrin alpha-3 light chain Integrin alpha 3 (antigen CD49C alpha 3 subunit of VLA-3 receptor) ITA3_HUMAN ITGA 3 Itga3 MSK18 VCA 2 VCA2 Very late activation protein 3 receptor alpha 3 subunit VL3A VLA 3 alpha chain VLA 3 subunit alpha VLA-3 subunit alpha VLA3a |
| Molecular Weight(MW) | 118kDa |
| Cellular Localization | Cell membrane,Cell projection, invadopodium membrane. |
WB
Western blot analysis of extracts from various samples, using Integrin alpha 3 Antibody. Lane 1: A549 cells, blocked with antigen-specific peptides, Lane 2: A549 cells, Lane 3: Hela cells(H2O2 treatment).
IHC
Integrin alpha 3 Antibody at 1/100 staining Human esophageal cancer and adjacent normal tissues by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4°C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.
ICC/IF
Integrin alpha 3 Antibody staining Hela by ICC/IF. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100,then blocked in 10% serum for 45 minutes at 25°C. Samples were then incubated with primary Ab(1:200) and mouse anti-beta tubulin Ab(1:200) for 1 hour at 37°C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) Ab(Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) Ab(Green) were used as the secondary antibody. The nuclear counter stain is DAPI(blue).| Application Notes | WB:1:500-1:2000 IHC:1:50-1:200 ICC/IF:1:100-1:500 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20 °C. Stable for 12 months from date of receipt. |
| Storage Buffer | Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
