WB
Various lysates were subjected to SDS PAGE followed by western blot with SFRP2 antibody at dilution of 1:3000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody as loading control.IHC
Immunohistochemical analysis of paraffin-embedded human malignant melanoma tissue slide using SFRP2 Antibody at dilution of 1:200 (under 10x lens).IHC
Immunohistochemical analysis of paraffin-embedded human malignant melanoma tissue slide using SFRP2 Antibody at dilution of 1:200 (under 40x lens).ICC/IF
Immunofluorescent analysis of (-20°C Methanol) fixed A549 cells using SFRP2 antibody at dilution of 1:1500 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).| Product Name | SFRP2 Monoclonal antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Recombinant human SFRP2 protein |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Host Species | Mouse |
| Tested Applications | ICC/IFIHCWB |
| WB:1:1000-1:6000 IHC:1:50-1:500 ICC/IF:1:750-1:3000 |
|
| Species Reactivity | HumanMousePigRabbitRat |
| Concentration | 1mg/ml |
| Purification | Protein G |
| Gene Symbol | SFRP2 |
|---|---|
| Gene Synonyms | FRP-2 SARP1 SDF-5 |
| Gene Full Name | secreted frizzled related protein 2 |
| Gene Summary | This gene encodes a member of the SFRP family that contains a cysteine-rich domain homologous to the putative Wnt-binding site of Frizzled proteins. SFRPs act as soluble modulators of Wnt signaling. Methylation of this gene is a potential marker for the presence of colorectal cancer. [provided by RefSeq, Jul 2008] |
| Alternative Names | 1F3A1 FKSG12 SARP1 SDF-5 Secreted apoptosis-related protein 1 |
| Molecular Weight(MW) | 34 kDa(Observed band size: 38 kDa) |
| Cellular Localization | Secreted. |
WB
Various lysates were subjected to SDS PAGE followed by western blot with SFRP2 antibody at dilution of 1:3000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody as loading control.
IHC
Immunohistochemical analysis of paraffin-embedded human malignant melanoma tissue slide using SFRP2 Antibody at dilution of 1:200 (under 10x lens).
IHC
Immunohistochemical analysis of paraffin-embedded human malignant melanoma tissue slide using SFRP2 Antibody at dilution of 1:200 (under 40x lens).
ICC/IF
Immunofluorescent analysis of (-20°C Methanol) fixed A549 cells using SFRP2 antibody at dilution of 1:1500 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).| Application Notes | WB:1:1000-1:6000 IHC:1:50-1:500 ICC/IF:1:750-1:3000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20°C. Stable for one year after shipment. |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
