WB
Western blot analysis of FXR1 on different lysates with Rabbit anti-FXR1 antibody at 1/1,000 dilution. Lane 1: HeLa cell lysate, Lane 2: 293T cell lysate, Lane 3: HepG2 cell lysate, Lane 4: A549 cell lysate, Lane 5: K-562 cell lysate, Lane 6: COS-1 cell lysate, Lane 7: NIH/3T3 cell lysate, Lane 8: C2C12 cell lysate, Lane 9: PC-12 cell lysate, Lysates/proteins at 30 µg/Lane. Exposure time: 3 minutes 10 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/100,000 dilution was used for 1 hour at room temperature.IHC
Immunohistochemical analysis of paraffin-embedded human testis tissue with Rabbit anti-FXR1 antibody at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.IHC
Immunohistochemical analysis of paraffin-embedded mouse brain tissue with Rabbit anti-FXR1 antibody at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.ICC/IF
Immunocytochemistry analysis of PC-12 cells labeling FXR1 with Rabbit anti-FXR1 antibody at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-FXR1 antibody at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (594) was used as the secondary antibody at 1/1,000 dilution.IP
FXR1 was immunoprecipitated from 0.2 mg HepG2 cell lysate with Rabbit anti-FXR1 antibody at 2 µg/10 µl beads. Western blot was performed from the immunoprecipitate using Rabbit anti-FXR1 antibody at 1/1,000 dilution. HRP Conjugated Anti-Rabbit IgG for IP Nano-secondary antibody at 1/5,000 dilution was used for 1 hour at room temperature. Lane 1: HepG2 cell lysate (input), Lane 2: Rabbit anti-FXR1 antibody IP in HepG2 cell lysate, Lane 3: Rabbit IgG instead ofRabbit anti-FXR1 antibody in HepG2 cell lysate, Blocking/Dilution buffer: primary antibody dilution, Exposure time: 10 seconds.| Product Name | FXR1 Recombinant Rabbit Monoclonal Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Recombinant protein within human FXR1 aa 1-150 / 621. |
| Clonality | monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHCIPWB |
| WB:1:1000 IHC:1:200 ICC/IF:1:100 IP:1-2μg/sample |
|
| Species Reactivity | HumanMonkeyMouseRat |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Symbol | FXR1 |
|---|---|
| Gene Synonyms | FXR1P CMYO9A CMYO9B CMYP9A CMYP9B MYOPMIL MYORIBF |
| Gene Full Name | FMR1 autosomal homolog 1 |
| Gene Summary | The protein encoded by this gene is an RNA binding protein that interacts with the functionally-similar proteins FMR1 and FXR2. These proteins shuttle between the nucleus and cytoplasm and associate with polyribosomes, predominantly with the 60S ribosomal subunit. Three transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008] |
| Alternative Names | Fragile X mental retardation autosomal homolog 1 antibody Fragile X mental retardation syndrome related protein 1 antibody Fragile X mental retardation syndrome-related protein 1 antibody FXR1 antibody FXR1_HUMAN antibody FXR1P antibody hFXR1p antibody |
| Molecular Weight(MW) | 70kDa(Observed band size: 70/75kDa) |
| Cellular Localization | Cytoplasm, Cytoplasmic ribonucleoprotein granule, Stress granule, Cell projection, dendrite, dendritic spine, axon, Nucleus envelope, Postsynapse. |
WB
Western blot analysis of FXR1 on different lysates with Rabbit anti-FXR1 antibody at 1/1,000 dilution. Lane 1: HeLa cell lysate, Lane 2: 293T cell lysate, Lane 3: HepG2 cell lysate, Lane 4: A549 cell lysate, Lane 5: K-562 cell lysate, Lane 6: COS-1 cell lysate, Lane 7: NIH/3T3 cell lysate, Lane 8: C2C12 cell lysate, Lane 9: PC-12 cell lysate, Lysates/proteins at 30 µg/Lane. Exposure time: 3 minutes 10 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/100,000 dilution was used for 1 hour at room temperature.
IHC
Immunohistochemical analysis of paraffin-embedded human testis tissue with Rabbit anti-FXR1 antibody at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
IHC
Immunohistochemical analysis of paraffin-embedded mouse brain tissue with Rabbit anti-FXR1 antibody at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ICC/IF
Immunocytochemistry analysis of PC-12 cells labeling FXR1 with Rabbit anti-FXR1 antibody at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-FXR1 antibody at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (594) was used as the secondary antibody at 1/1,000 dilution.
IP
FXR1 was immunoprecipitated from 0.2 mg HepG2 cell lysate with Rabbit anti-FXR1 antibody at 2 µg/10 µl beads. Western blot was performed from the immunoprecipitate using Rabbit anti-FXR1 antibody at 1/1,000 dilution. HRP Conjugated Anti-Rabbit IgG for IP Nano-secondary antibody at 1/5,000 dilution was used for 1 hour at room temperature. Lane 1: HepG2 cell lysate (input), Lane 2: Rabbit anti-FXR1 antibody IP in HepG2 cell lysate, Lane 3: Rabbit IgG instead ofRabbit anti-FXR1 antibody in HepG2 cell lysate, Blocking/Dilution buffer: primary antibody dilution, Exposure time: 10 seconds.| Application Notes | WB:1:1000 IHC:1:200 ICC/IF:1:100 IP:1-2μg/sample |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
