WB
Western blot analysis of lysates from HeLa cells, using Phospho-STAT2-Y690 Rabbit pAb at 1:500 dilution.HeLa cells were treated by IFN-α (100 ng/ml) at 37℃ for 30 minutes after serum-starvation overnight. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.| Product Name | Phospho-STAT2-Y690 Rabbit pAb |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | A synthetic phosphorylated peptide around Y690 of human STAT2 (NP_005410.1). |
| Modification | P-Y690 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB:1:100-1:500 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | STAT2 |
|---|---|
| Gene Synonyms | P113 IMD44 ISGF-3 PTORCH3 STAT113 |
| Gene Full Name | signal transducer and activator of transcription 2 |
| Gene Summary | The protein encoded by this gene is a member of the STAT protein family. In response to cytokines and growth factors, STAT family members are phosphorylated by the receptor associated kinases, and then form homo- or heterodimers that translocate to the cell nucleus where they act as transcription activators. In response to interferon (IFN), this protein forms a complex with STAT1 and IFN regulatory factor family protein p48 (ISGF3G), in which this protein acts as a transactivator, but lacks the ability to bind DNA directly. The protein mediates innate antiviral activity. Mutations in this gene result in Immunodeficiency 44. [provided by RefSeq, Aug 2020] |
| Molecular Weight(MW) | 97kDa |
| Cellular Localization | Cytoplasm, Nucleus. |
WB
Western blot analysis of lysates from HeLa cells, using Phospho-STAT2-Y690 Rabbit pAb at 1:500 dilution.HeLa cells were treated by IFN-α (100 ng/ml) at 37℃ for 30 minutes after serum-starvation overnight. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.| Application Notes | WB:1:100-1:500 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20℃. Avoid freeze / thaw cycles. |
| Storage Buffer | PBS with 0.01% thimerosal, 50% glycerol, pH7.3. |
