WB
Western blot analysis of extracts of various cell lines, using SNAP25 Rabbit mAb at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.| Product Name | SNAP25 Rabbit mAb |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | A synthetic peptide corresponding to a sequence within amino acids 107-206 of human SNAP25 (P60880). |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB:1:500-1:1000 |
|
| Species Reactivity | MouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | SNAP25 |
|---|---|
| Gene Synonyms | SUP RIC4 SEC9 SNAP CMS18 RIC-4 DEE117 SNAP-25 bA416N4.2 dJ1068F16.2 |
| Gene Full Name | synaptosome associated protein 25 |
| Gene Summary | Synaptic vesicle membrane docking and fusion is mediated by SNAREs (soluble N-ethylmaleimide-sensitive factor attachment protein receptors) located on the vesicle membrane (v-SNAREs) and the target membrane (t-SNAREs). The assembled v-SNARE/t-SNARE complex consists of a bundle of four helices, one of which is supplied by v-SNARE and the other three by t-SNARE. For t-SNAREs on the plasma membrane, the protein syntaxin supplies one helix and the protein encoded by this gene contributes the other two. Therefore, this gene product is a presynaptic plasma membrane protein involved in the regulation of neurotransmitter release. Two alternative transcript variants encoding different protein isoforms have been described for this gene. [provided by RefSeq, Jul 2008] |
| Molecular Weight(MW) | 25kDa |
| Cellular Localization | Cell junction, Cell membrane, Cytoplasm, Lipid-anchor, perinuclear region, synapse, synaptosome. |
WB
Western blot analysis of extracts of various cell lines, using SNAP25 Rabbit mAb at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.| Application Notes | WB:1:500-1:1000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20℃. Avoid freeze / thaw cycles. |
| Storage Buffer | PBS with 0.02% sodium azide, 0.05% BSA, 50% glycerol, pH7.3. |
