WB
fetal human brain tissue were subjected to SDS PAGE followed by western blot with SNAP25 antibody at dilution of 1:2000 incubated at room temperature for 1.5 hours.WB
mouse brain tissue were subjected to SDS PAGE followed by western blot with SNAP25 antibody at dilution of 1:10000 incubated at room temperature for 1.5 hours.WB
rat brain tissue were subjected to SDS PAGE followed by western blot with SNAP25 antibody at dilution of 1:10000 incubated at room temperature for 1.5 hours.IHC
Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using SNAP25 antibody at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).IHC
Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using SNAP25 antibody at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).ICC/IF
Immunofluorescent analysis of (-20°C Ethanol) fixed PC-12 cells using SNAP25 antibody at dilution of 1:1000 and CoraLite®594-Conjugated Goat Anti-Mouse IgG(H+L).IP
IP result of anti-SNAP25 (IP:3ug; Detection:1:500) with mouse brain tissue lysate 3600ug.| Product Name | SNAP25 Monoclonal antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Recombinant human SNAP protein |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG2b |
| Host Species | Mouse |
| Tested Applications | ICC/IFIHCIPWB |
| WB:1:5000-1:20000 IHC:1:1000-1:4000 ICC/IF:1:200-1:800 IP: 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | SNAP25 |
|---|---|
| Gene Synonyms | SUP RIC4 SEC9 SNAP CMS18 RIC-4 DEE117 SNAP-25 bA416N4.2 dJ1068F16.2 |
| Gene Full Name | synaptosome associated protein 25 |
| Gene Summary | Synaptic vesicle membrane docking and fusion is mediated by SNAREs (soluble N-ethylmaleimide-sensitive factor attachment protein receptors) located on the vesicle membrane (v-SNAREs) and the target membrane (t-SNAREs). The assembled v-SNARE/t-SNARE complex consists of a bundle of four helices, one of which is supplied by v-SNARE and the other three by t-SNARE. For t-SNAREs on the plasma membrane, the protein syntaxin supplies one helix and the protein encoded by this gene contributes the other two. Therefore, this gene product is a presynaptic plasma membrane protein involved in the regulation of neurotransmitter release. Two alternative transcript variants encoding different protein isoforms have been described for this gene. [provided by RefSeq, Jul 2008] |
| Alternative Names | 3E4B7 RIC 4 RIC4 RIC-4 SNAP |
| Molecular Weight(MW) | 25-30 kDa |
| Cellular Localization | Cell membrane,Cytoplasm,Membrane,Synapse,Synaptosome. |
WB
fetal human brain tissue were subjected to SDS PAGE followed by western blot with SNAP25 antibody at dilution of 1:2000 incubated at room temperature for 1.5 hours.
WB
mouse brain tissue were subjected to SDS PAGE followed by western blot with SNAP25 antibody at dilution of 1:10000 incubated at room temperature for 1.5 hours.
WB
rat brain tissue were subjected to SDS PAGE followed by western blot with SNAP25 antibody at dilution of 1:10000 incubated at room temperature for 1.5 hours.
IHC
Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using SNAP25 antibody at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC
Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using SNAP25 antibody at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
ICC/IF
Immunofluorescent analysis of (-20°C Ethanol) fixed PC-12 cells using SNAP25 antibody at dilution of 1:1000 and CoraLite®594-Conjugated Goat Anti-Mouse IgG(H+L).
IP
IP result of anti-SNAP25 (IP:3ug; Detection:1:500) with mouse brain tissue lysate 3600ug.| Application Notes | WB:1:5000-1:20000 IHC:1:1000-1:4000 ICC/IF:1:200-1:800 IP: 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20°C. Stable for one year after shipment. |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
