WB
ATP1A1/ATP1A2/ATP1A3 antibodies: . Direct Western blot analysis of Na+/K+-ATPase α expression in HeLa (A) and C6 (B) whole cell lysatesWB
Na+/K+-ATPase α antibodies: Western blot analysis of Na+/K+-ATPase α expression in HeLa (A), Jurkat (B), EOC20 (C) and NIH/3T3 (D) whole cell lysates.IHC
Na+/K+-ATPase α : Immunoperoxidase staining of formalin-fixed, paraffin-embedded human kidney tissue showed cytoplasmic staining of glomerular cells and membrane and cytoplasmic staining of renal tubular cells.ICC/IF
Na+/K+-ATPase α : Immunofluorescent staining of methanol-fixed HeLa cells showed membrane localization.WB
Na+/K+-ATPase α antibodies: Direct Western blot analysis was performed on the expression of Na+/K+-ATPase α in HeLa (A) and Hep G2 (B) whole cell lysates and mouse placenta (C), human kidney (D) and human brain (E) tissue extracts.WB
Na+/K+-ATPase α : Western blot analysis of Na+/K+-ATPase α1 expression in whole cell lysates of untransfected 293T (A), transfected 293T (B) and HeLa (C).| Product Name | Mouse Monoclonal antibody to ATP1A1/ATP1A2/ATP1A3 |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | amino acids 551-850 of Na+/K+-ATPase a1 of human origin origin. |
| Clonality | monoclonal |
|---|---|
| Isotype | IgG2b,k |
| Host Species | Mouse |
| Tested Applications | ELISAICC/IFIHCWB |
| WB:1:100-1:1000 ICC/IF:1:50-1:500 IHC:1:50-1:500 ELISA:1:1000-1:10000 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 0.2mg/ml |
| Purification | Affinity purified |
| Gene Symbol | ATP1A1 |
|---|---|
| Gene Synonyms | CMT2DD HOMGSMR2 |
| Gene Full Name | ATPase Na+/K+ transporting subunit alpha 1 |
| Gene Summary | The protein encoded by this gene belongs to the family of P-type cation transport ATPases, and to the subfamily of Na+/K+ -ATPases. Na+/K+ -ATPase is an integral membrane protein responsible for establishing and maintaining the electrochemical gradients of Na and K ions across the plasma membrane. These gradients are essential for osmoregulation, for sodium-coupled transport of a variety of organic and inorganic molecules, and for electrical excitability of nerve and muscle. This enzyme is composed of two subunits, a large catalytic subunit (alpha) and a smaller glycoprotein subunit (beta). The catalytic subunit of Na+/K+ -ATPase is encoded by multiple genes. This gene encodes an alpha 1 subunit. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, May 2009] |
| Molecular Weight(MW) | Molecular Weight of Na+/K+-ATPase α isoforms: 100-113 kDa. |
| Cellular Localization | Cell membrane ;Cell projection;Melanosome |
WB
ATP1A1/ATP1A2/ATP1A3 antibodies: . Direct Western blot analysis of Na+/K+-ATPase α expression in HeLa (A) and C6 (B) whole cell lysates
WB
Na+/K+-ATPase α antibodies: Western blot analysis of Na+/K+-ATPase α expression in HeLa (A), Jurkat (B), EOC20 (C) and NIH/3T3 (D) whole cell lysates.
IHC
Na+/K+-ATPase α : Immunoperoxidase staining of formalin-fixed, paraffin-embedded human kidney tissue showed cytoplasmic staining of glomerular cells and membrane and cytoplasmic staining of renal tubular cells.
ICC/IF
Na+/K+-ATPase α : Immunofluorescent staining of methanol-fixed HeLa cells showed membrane localization.
WB
Na+/K+-ATPase α antibodies: Direct Western blot analysis was performed on the expression of Na+/K+-ATPase α in HeLa (A) and Hep G2 (B) whole cell lysates and mouse placenta (C), human kidney (D) and human brain (E) tissue extracts.
WB
Na+/K+-ATPase α : Western blot analysis of Na+/K+-ATPase α1 expression in whole cell lysates of untransfected 293T (A), transfected 293T (B) and HeLa (C).| Application Notes | WB:1:100-1:1000 ICC/IF:1:50-1:500 IHC:1:50-1:500 ELISA:1:1000-1:10000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4° C, **DO NOT FREEZE**. Stable for one year from the date of shipment. Non-hazardous. No MSDS required. |
| Storage Buffer | Each vial contains 200 µg IgG1 kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin. |
