WB
Western blot analysis of extracts from RAW264.7 cells(serum starvation treatment), using Phospho-ULK1 (Ser757)Antibody. The lane on the left was treated with blocking peptide.IHC
Phospho-ULK1 (Ser757)Antibody at 1/100 staining Rat heart tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4°C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.IHC
Phospho-ULK1 (Ser757)Antibody at 1/100 staining Rat skin tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4°C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.| Product Name | Rabbit polyclonal antibody to Phospho-ULK1 (Ser757) |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | A synthesized peptide derived from mouse ULK1(Accession O75385), corresponding to amino acid residues around phosphorylated Ser758. |
| Modification | P-Ser757 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IHCWB |
| WB:1:500-1:2000 IHC:1:50-1:200 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | ULK1 |
|---|---|
| Gene Synonyms | ATG1 ATG1A UNC51 hATG1 Unc51.1 |
| Gene Full Name | unc-51 like autophagy activating kinase 1 |
| Gene Summary | Enables identical protein binding activity; protein serine/threonine kinase activity; and small GTPase binding activity. Involved in several processes, including autophagosome assembly; protein phosphorylation; and regulation of autophagy. Located in bounding membrane of organelle; cytosol; and phagophore assembly site membrane. Part of Atg1/ULK1 kinase complex. Is active in cytoplasm. [provided by Alliance of Genome Resources, Jun 2025] |
| Alternative Names | ATG 1 ATG1 ATG1 autophagy related 1 homolog ATG1A Autophagy related protein 1 homolog Autophagy-related protein 1 homolog FLJ38455 FLJ46475 hATG1 KIAA0722 Serine/threonine protein kinase ULK1 Serine/threonine protein kinase Unc51.1 Serine/threonine-protein kinase ULK1 ULK 1 ULK1 ULK1_HUMAN Unc 51 (C. elegans) like kinase 1 UNC 51 Unc 51 like kinase 1 Unc-51 like kinase 1 (C. elegans) Unc-51-like kinase 1 UNC51 UNC51 C. elegans homolog of Unc51.1 |
| Molecular Weight(MW) | 113kDa(Observed MW 120-150kDa) |
| Cellular Localization | Cytoplasm>Cytosol. Preautophagosomal structure. |
WB
Western blot analysis of extracts from RAW264.7 cells(serum starvation treatment), using Phospho-ULK1 (Ser757)Antibody. The lane on the left was treated with blocking peptide.
IHC
Phospho-ULK1 (Ser757)Antibody at 1/100 staining Rat heart tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4°C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.
IHC
Phospho-ULK1 (Ser757)Antibody at 1/100 staining Rat skin tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4°C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.| Application Notes | WB:1:500-1:2000 IHC:1:50-1:200 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20 °C. Stable for 12 months from date of receipt. |
| Storage Buffer | Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
