DB
Dot blot analysis was performed using Anti-Phospho-ULK1 (Ser758) antibody to detect two peptide samples: lane 1 contains the phospho-peptide , and lane 2 contains the non-phospho-peptide. Transfer the proteins onto a NC membrane (OM790001), and block it with TBST (OM750016) containing 5% BSA(OM297470) for 1 hour. Dilute the primary antibody with the antibody diluent (OM750012) at a ratio of 1:1000, and incubate it at room temperature for 2 hours. Wash the membrane 3 times with TBST (OM750016), 5 minutes each time. At room temperature, dilute the secondary antibody, Goat Anti-Rabbit IgG(H&L)-HRP (OM643487), at a ratio of 1:10000 and incubate for 1 hour. Wash the membrane 3 times with TBST (OM750016) again, 5 minutes each time. Use ECL (OM625701) for luminescence. Exposure time: 30 s.| Product Name | Anti-Phospho-ULK1 (Ser758) antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Synthetic peptide conjugated with KLH. |
| Modification | p-Ser758 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | DB |
| DB:1:1000 |
|
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | ULK1 |
|---|---|
| Gene Synonyms | ATG1 ATG1A UNC51 hATG1 Unc51.1 |
| Gene Full Name | unc-51 like autophagy activating kinase 1 |
| Gene Summary | Enables identical protein binding activity; protein serine/threonine kinase activity; and small GTPase binding activity. Involved in several processes, including autophagosome assembly; protein phosphorylation; and regulation of autophagy. Located in bounding membrane of organelle; cytosol; and phagophore assembly site membrane. Part of Atg1/ULK1 kinase complex. Is active in cytoplasm. [provided by Alliance of Genome Resources, Jul 2025] |
| Alternative Names | ATG 1 ATG1 ATG1 autophagy related 1 homolog ATG1A Autophagy related protein 1 homolog Autophagy-related protein 1 homolog FLJ38455 FLJ46475 hATG1 KIAA0722 Serine/threonine protein kinase ULK1 Serine/threonine protein kinase Unc51.1 Serine/threonine-protein kinase ULK1 ULK 1 ULK1 ULK1_HUMAN Unc 51 (C. elegans) like kinase 1 UNC 51 Unc 51 like kinase 1 Unc-51 like kinase 1 (C. elegans) Unc-51-like kinase 1 UNC51 UNC51 C. elegans homolog of Unc51.1 |
| Molecular Weight(MW) | 113kDa |
| Cellular Localization | Cytoplasm |
| SwissProt ID | O75385 |
|---|
DB
Dot blot analysis was performed using Anti-Phospho-ULK1 (Ser758) antibody to detect two peptide samples: lane 1 contains the phospho-peptide , and lane 2 contains the non-phospho-peptide. Transfer the proteins onto a NC membrane (OM790001), and block it with TBST (OM750016) containing 5% BSA(OM297470) for 1 hour. Dilute the primary antibody with the antibody diluent (OM750012) at a ratio of 1:1000, and incubate it at room temperature for 2 hours. Wash the membrane 3 times with TBST (OM750016), 5 minutes each time. At room temperature, dilute the secondary antibody, Goat Anti-Rabbit IgG(H&L)-HRP (OM643487), at a ratio of 1:10000 and incubate for 1 hour. Wash the membrane 3 times with TBST (OM750016) again, 5 minutes each time. Use ECL (OM625701) for luminescence. Exposure time: 30 s.| Application Notes | DB:1:1000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Storage Buffer | Purified antibody in PBS with 0.05% sodium azide,40% Glycerol. |
