WB
HeLa cells were subjected to SDS PAGE followed by western blot with LSM5 antibody at dilution of 1:100 incubated at room temperature for 1.5 hours.WB
Various lysates were subjected to SDS PAGE followed by western blot with LSM5 antibody at dilution of 1:600 incubated at room temperature for 1.5 hours.IHC
Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using LSM5 antibody at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).ICC/IF
Immunofluorescent analysis of (10% Formaldehyde) fixed HeLa cells using LSM5 antibody at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).| Product Name | LSM5 Polyclonal antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Recombinant human LSM5 protein. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHCWB |
| WB:1:200-1:1000 IHC:1:250-1:1000 ICC/IF:1:50-1:500 |
|
| Species Reactivity | HumanMouse |
| Concentration | 0.8mg/ml |
| Purification | Affinity purified |
| Gene Symbol | LSM5 |
|---|---|
| Gene Synonyms | YER146W |
| Gene Full Name | LSM5 homolog, U6 small nuclear RNA and mRNA degradation associated |
| Gene Summary | Sm-like proteins were identified in a variety of organisms based on sequence homology with the Sm protein family (see SNRPD2; MIM 601061). Sm-like proteins contain the Sm sequence motif, which consists of 2 regions separated by a linker of variable length that folds as a loop. The Sm-like proteins are thought to form a stable heteromer present in tri-snRNP particles, which are important for pre-mRNA splicing.[supplied by OMIM, Apr 2004] |
| Alternative Names | YER146W U6 snRNA-associated Sm-like protein LSm5 |
| Molecular Weight(MW) | 10kDa(Observed MW:12kDa) |
| Cellular Localization | Nucleus |
WB
HeLa cells were subjected to SDS PAGE followed by western blot with LSM5 antibody at dilution of 1:100 incubated at room temperature for 1.5 hours.
WB
Various lysates were subjected to SDS PAGE followed by western blot with LSM5 antibody at dilution of 1:600 incubated at room temperature for 1.5 hours.
IHC
Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using LSM5 antibody at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
ICC/IF
Immunofluorescent analysis of (10% Formaldehyde) fixed HeLa cells using LSM5 antibody at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).| Application Notes | WB:1:200-1:1000 IHC:1:250-1:1000 ICC/IF:1:50-1:500 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20°C. Stable for one year after shipment. |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
