WB
Western blot analysis of Phospho-PI3K p85 (Y467) + PI3K p55 (Y199) on different lysates with Rabbit anti-Phospho-PI3K p85 (Y467) + PI3K p55 (Y199) antibody at 1/1,000 dilution. Lane 1: NIH/3T3 cell lysate, Lane 2: NIH/3T3 treated with 1mM sodium orthovanadate for 30 minutes cell lysate, Lysates/proteins at 30 µg/Lane. Exposure time: 3 minutes 10 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/100,000 dilution was used for 1 hour at room temperature.WB
Western blot analysis of Phospho-PI3K p85 (Y467) + PI3K p55 (Y199) on different lysates with Rabbit anti-Phospho-PI3K p85 (Y467) + PI3K p55 (Y199) antibody at 1/1,000 dilution. Lane 1: 293T overexpress Src cell lysate, Lane 2: 293T overexpress Src treated with 1mM sodium orthovanadate for 30 minutes cell lysate, Lane 3: 293T overexpress Src cell lysate, the membrane treated with λpp for 1 hour, Lane 4: 293T overexpress Src treated with 1mM sodium orthovanadate for 30 minutes cell lysate, then the membrane treated with λpp for 1 hour. Lysates/proteins at 20 µg/Lane. Exposure time: 28 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/100,000 dilution was used for 1 hour at room temperature.ICC/IF
Immunocytochemistry analysis of NIH/3T3 cells treated with or without 1mM Sodium orthovanadate for 30 minutes labeling Phospho-PI3K p85 (Y467) + PI3K p55 (Y199) with Rabbit anti-Phospho-PI3K p85 (Y467) + PI3K p55 (Y199) antibody at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-Phospho-PI3K p85 (Y467) + PI3K p55 (Y199) antibody at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (594) was used as the secondary antibody at 1/1,000 dilution.| Product Name | Phospho-PI3K p85 (Y467) + PI3K p55 (Y199) Recombinant Rabbit Monoclonal Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Synthetic peptide within human PIK3R1 aa 451-500 / 724. |
| Modification | p-PI3K p85 (Y467) + PI3K p55 (Y199) |
| Clonality | monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFWB |
| WB:1:1000 ICC/IF:1:100 |
|
| Species Reactivity | HumanMouse |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Symbol | PIK3R1 |
|---|---|
| Gene Synonyms | p85 AGM7 GRB1 IMD36 p85alpha p85-ALPHA |
| Gene Full Name | phosphoinositide-3-kinase regulatory subunit 1 |
| Gene Summary | Phosphatidylinositol 3-kinase phosphorylates the inositol ring of phosphatidylinositol at the 3-prime position. The enzyme comprises a 110 kD catalytic subunit and a regulatory subunit of either 85, 55, or 50 kD. This gene encodes the 85 kD regulatory subunit. Phosphatidylinositol 3-kinase plays an important role in the metabolic actions of insulin, and a mutation in this gene has been associated with insulin resistance. Alternative splicing of this gene results in four transcript variants encoding different isoforms. [provided by RefSeq, Jun 2011] |
| Alternative Names | GRB1 antibody p85 alpha antibody p85 antibody P85A_HUMAN antibody Phosphatidylinositol 3 kinase associated p 85 alpha antibody Phosphatidylinositol 3 kinase regulatory 1 antibody Phosphatidylinositol 3 kinase regulatory subunit polypeptide 1 (p85 alpha) antibody Phosphatidylinositol 3-kinase 85 kDa regulatory subunit alpha antibody Phosphatidylinositol 3-kinase regulatory subunit alpha antibody Phosphoinositide 3 kinase regulatory subunit 1 (alpha) antibody GRB1 antibody p85 alpha antibody p85 antibody P85A_HUMAN antibody Phosphatidylinositol 3 kinase associated p 85 alpha antibody Phosphatidylinositol 3 kinase regulatory 1 antibody Phosphatidylinositol 3 kinase regulatory subunit polypeptide 1 (p85 alpha) antibody Phosphatidylinositol 3-kinase 85 kDa regulatory subunit alpha antibody Phosphatidylinositol 3-kinase regulatory subunit alpha antibody Phosphoinositide 3 kinase regulatory subunit 1 (alpha) antibody PI3 kinase p85 antibody PI3 kinase p85 subunit alpha antibody PI3-kinase regulatory subunit alpha antibody PI3-kinase subunit p85-alpha antibody PI3K antibody PI3K p85 antibody PI3K regulatory subunit alpha antibody Pik3r1 antibody PtdIns 3 kinase p85 alpha antibody PtdIns-3-kinase regulatory subunit alpha antibody PtdIns-3-kinase regulatory subunit p85-alpha antibody |
| Molecular Weight(MW) | 84kDa(Observed band size: 55/85kDa) |
| Cellular Localization | Cell-cell junction, cis-Golgi network, cytoplasm, cytosol, membrane, nucleus. |
WB
Western blot analysis of Phospho-PI3K p85 (Y467) + PI3K p55 (Y199) on different lysates with Rabbit anti-Phospho-PI3K p85 (Y467) + PI3K p55 (Y199) antibody at 1/1,000 dilution. Lane 1: NIH/3T3 cell lysate, Lane 2: NIH/3T3 treated with 1mM sodium orthovanadate for 30 minutes cell lysate, Lysates/proteins at 30 µg/Lane. Exposure time: 3 minutes 10 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/100,000 dilution was used for 1 hour at room temperature.
WB
Western blot analysis of Phospho-PI3K p85 (Y467) + PI3K p55 (Y199) on different lysates with Rabbit anti-Phospho-PI3K p85 (Y467) + PI3K p55 (Y199) antibody at 1/1,000 dilution. Lane 1: 293T overexpress Src cell lysate, Lane 2: 293T overexpress Src treated with 1mM sodium orthovanadate for 30 minutes cell lysate, Lane 3: 293T overexpress Src cell lysate, the membrane treated with λpp for 1 hour, Lane 4: 293T overexpress Src treated with 1mM sodium orthovanadate for 30 minutes cell lysate, then the membrane treated with λpp for 1 hour. Lysates/proteins at 20 µg/Lane. Exposure time: 28 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/100,000 dilution was used for 1 hour at room temperature.
ICC/IF
Immunocytochemistry analysis of NIH/3T3 cells treated with or without 1mM Sodium orthovanadate for 30 minutes labeling Phospho-PI3K p85 (Y467) + PI3K p55 (Y199) with Rabbit anti-Phospho-PI3K p85 (Y467) + PI3K p55 (Y199) antibody at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-Phospho-PI3K p85 (Y467) + PI3K p55 (Y199) antibody at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (594) was used as the secondary antibody at 1/1,000 dilution.| Application Notes | WB:1:1000 ICC/IF:1:100 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
