WB
Western Blot analysis of KB cells using Phospho-PI 3-kinase p85α (Y607) Polyclonal Antibody diluted at 1:1000.WB
Western blot analysis of lysates from rat kidney, using PI3-kinase p85-alpha (Phospho-Tyr607) Antibody. The lane on the right is blocked with the phospho peptide.IHC
Immunohistochemical analysis of paraffin-embedded Mouse-colon tissue. 1,PI 3-kinase p85α (phospho Tyr607) Polyclonal Antibody was diluted at 1:200(4°C,overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98°C,20min). 3,Secondary antibody was diluted at 1:200(room tempeRature, 30min). Negative control was used by secondary antibody only.IHC
Immunohistochemical analysis of paraffin-embedded Human-liver-cancer tissue. 1,PI 3-kinase p85α (phospho Tyr607) Polyclonal Antibody was diluted at 1:200(4°C,overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98°C,20min). 3,Secondary antibody was diluted at 1:200(room tempeRature, 30min). Negative control was used by secondary antibody only.IHC
Immunohistochemical analysis of paraffin-embedded Rat-heart tissue. 1,PI 3-kinase p85α (phospho Tyr607) Polyclonal Antibody was diluted at 1:200(4°C,overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98°C,20min). 3,Secondary antibody was diluted at 1:200(room tempeRature, 30min). Negative control was used by secondary antibody only.ICC/IF
Immunofluorescence analysis of Siha cell. 1,primary Antibody was diluted at 1:100(4°C overnight). 2, Goat Anti Rabbit IgG (H&L) - AFluor 594 Secondary antibody was diluted at 1:500(room temperature, 50min).| Product Name | PI 3 kinase p85α (Phospho Tyr607) Rabbit pAb |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | The antiserum was produced against synthesized peptide derived from human PI3-kinase p85-alpha around the phosphorylation site of Tyr607. AA range:573-622 |
| Modification | p-Tyr607 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHCWB |
| WB:1:500-1:2000 IHC:100-1:300 ICC/IF:1:50-1:200 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | PIK3R1 |
|---|---|
| Gene Synonyms | p85 AGM7 GRB1 IMD36 p85alpha p85-ALPHA |
| Gene Full Name | phosphoinositide-3-kinase regulatory subunit 1 |
| Gene Summary | Phosphatidylinositol 3-kinase phosphorylates the inositol ring of phosphatidylinositol at the 3-prime position. The enzyme comprises a 110 kD catalytic subunit and a regulatory subunit of either 85, 55, or 50 kD. This gene encodes the 85 kD regulatory subunit. Phosphatidylinositol 3-kinase plays an important role in the metabolic actions of insulin, and a mutation in this gene has been associated with insulin resistance. Alternative splicing of this gene results in four transcript variants encoding different isoforms. [provided by RefSeq, Jun 2011] |
| Alternative Names | PIK3R1 GRB1 Phosphatidylinositol 3-kinase regulatory subunit alpha PI3-kinase regulatory subunit alpha PI3K regulatory subunit alpha PtdIns-3-kinase regulatory subunit alpha Phosphatidylinositol 3-kinase 85 kDa regulatory subunit alpha PI3-kinase subunit p85-alpha PtdIns-3-kinase regulatory subunit p85-alpha |
| Molecular Weight(MW) | 85kDa |
| Cellular Localization | Cell-cell junction, cis-Golgi network, cytoplasm, cytosol, membrane, nucleus. |
WB
Western Blot analysis of KB cells using Phospho-PI 3-kinase p85α (Y607) Polyclonal Antibody diluted at 1:1000.
WB
Western blot analysis of lysates from rat kidney, using PI3-kinase p85-alpha (Phospho-Tyr607) Antibody. The lane on the right is blocked with the phospho peptide.
IHC
Immunohistochemical analysis of paraffin-embedded Mouse-colon tissue. 1,PI 3-kinase p85α (phospho Tyr607) Polyclonal Antibody was diluted at 1:200(4°C,overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98°C,20min). 3,Secondary antibody was diluted at 1:200(room tempeRature, 30min). Negative control was used by secondary antibody only.
IHC
Immunohistochemical analysis of paraffin-embedded Human-liver-cancer tissue. 1,PI 3-kinase p85α (phospho Tyr607) Polyclonal Antibody was diluted at 1:200(4°C,overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98°C,20min). 3,Secondary antibody was diluted at 1:200(room tempeRature, 30min). Negative control was used by secondary antibody only.
IHC
Immunohistochemical analysis of paraffin-embedded Rat-heart tissue. 1,PI 3-kinase p85α (phospho Tyr607) Polyclonal Antibody was diluted at 1:200(4°C,overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98°C,20min). 3,Secondary antibody was diluted at 1:200(room tempeRature, 30min). Negative control was used by secondary antibody only.
ICC/IF
Immunofluorescence analysis of Siha cell. 1,primary Antibody was diluted at 1:100(4°C overnight). 2, Goat Anti Rabbit IgG (H&L) - AFluor 594 Secondary antibody was diluted at 1:500(room temperature, 50min).| Application Notes | WB:1:500-1:2000 IHC:100-1:300 ICC/IF:1:50-1:200 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | -15°C to -25°C/1 year(Do not lower than -25°C) |
| Storage Buffer | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
