WB
Positive WB detected in: Hela whole cell lysate, Mouse liver tissue, Mouse kidney tissue, All lanes: EHD2 antibody at 3μg/ml, Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution.IHC
Immunohistochemistry of paraffin-embedded human kidney tissue using EHD2 Antibody at dilution of 1:100.IF
Immunofluorescent analysis of Hela cells using EHD2 Antibody at dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).| Product Name | Rabbit anti-EHD2 Polyclonal antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Recombinant Human EH domain-containing protein 2 protein (401-543AA). |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHCWB |
| WB:1:500-1:5000 IHC:1:20-1:200 ICC/IF:1:50-1:200 |
|
| Species Reactivity | HumanMouse |
| Concentration | 1mg/ml |
| Purification | Protein G |
| Gene Symbol | EHD2 |
|---|---|
| Gene Synonyms | PAST2 |
| Gene Full Name | EH domain containing 2 |
| Gene Summary | This gene encodes a member of the EH domain-containing protein family. These proteins are characterized by a C-terminal EF-hand domain, a nucleotide-binding consensus site at the N terminus and a bipartite nuclear localization signal. The encoded protein interacts with the actin cytoskeleton through an N-terminal domain and also binds to an EH domain-binding protein through the C-terminal EH domain. This interaction appears to connect clathrin-dependent endocytosis to actin, suggesting that this gene product participates in the endocytic pathway. [provided by RefSeq, Jul 2008] |
| Alternative Names | EH domain containing 2 antibody EH domain containing 3 antibody EH domain containing protein 2 antibody EH domain-containing protein 2 antibody EHD 2 antibody Ehd2 antibody EHD2_HUMAN antibody EHD3 antibody FLJ96617 antibody PAST 2 antibody PAST homolog 2 antibody PAST2 antibody |
| Molecular Weight(MW) | 62kDa,47kDa |
| Cellular Localization | Cell membrane; Peripheral membrane protein; Cytoplasmic side. Membrane, caveola; Peripheral membrane protein; Cytoplasmic side. Endosome membrane; Peripheral membrane protein; Cytoplasmic side. Cytoplasm, cytosol. |
WB
Positive WB detected in: Hela whole cell lysate, Mouse liver tissue, Mouse kidney tissue, All lanes: EHD2 antibody at 3μg/ml, Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution.
IHC
Immunohistochemistry of paraffin-embedded human kidney tissue using EHD2 Antibody at dilution of 1:100.
IF
Immunofluorescent analysis of Hela cells using EHD2 Antibody at dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).| Application Notes | WB:1:500-1:5000 IHC:1:20-1:200 ICC/IF:1:50-1:200 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Upon receipt, store at -20°C or -80°C. Avoid repeated freeze. |
| Storage Buffer | Preservative: 0.03% Proclin 300,Constituents: 50% Glycerol, 0.01M PBS, pH 7.4 |
