WB
HeLa cells were subjected to SDS PAGE followed by western blot with PTGDS antibody at dilution of 1:500 incubated at room temperature for 1.5 hours.WB
Various lysates were subjected to SDS PAGE followed by western blot with PTGDS antibody at dilution of 1:1500 incubated at room temperature for 1.5 hours.IHC
Immunohistochemical analysis of paraffin-embedded human lung cancer using PTGDS antibody at dilution of 1:50 (under 40x lens).IHC
Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using PTGDS antibody at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).IP
IP result of anti-PTGDS (IP: 4ug; Detection:1:600) with mouse brain tissue lysate 1.8mg.| Product Name | PTGDS Polyclonal antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Recombinant human PGDS protein. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IHCIPWB |
| WB:1:500-1:3000 IHC:1:250-1:1000 IP:0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
|
| Species Reactivity | HumanMouse |
| Concentration | 0.5mg/ml |
| Purification | Affinity purified |
| Gene Symbol | PTGDS |
|---|---|
| Gene Synonyms | PDS PGD2 PGDS LPGDS PGDS2 L-PGDS |
| Gene Full Name | prostaglandin D2 synthase |
| Gene Summary | The protein encoded by this gene is a glutathione-independent prostaglandin D synthase that catalyzes the conversion of prostaglandin H2 (PGH2) to postaglandin D2 (PGD2). PGD2 functions as a neuromodulator as well as a trophic factor in the central nervous system. PGD2 is also involved in smooth muscle contraction/relaxation and is a potent inhibitor of platelet aggregation. This gene is preferentially expressed in brain. Studies with transgenic mice overexpressing this gene suggest that this gene may be also involved in the regulation of non-rapid eye movement sleep. [provided by RefSeq, Jul 2008] |
| Alternative Names | LPGDS Lipocalin-type prostaglandin-D synthase Glutathione-independent PGD synthase EC:5.3.99.2 Cerebrin-28 |
| Molecular Weight(MW) | 21kDa(Observed MW:28kDa) |
| Cellular Localization | Cytoplasm,Endoplasmic reticulum,Golgi apparatus,Membrane,Nucleus,Secreted |
WB
HeLa cells were subjected to SDS PAGE followed by western blot with PTGDS antibody at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB
Various lysates were subjected to SDS PAGE followed by western blot with PTGDS antibody at dilution of 1:1500 incubated at room temperature for 1.5 hours.
IHC
Immunohistochemical analysis of paraffin-embedded human lung cancer using PTGDS antibody at dilution of 1:50 (under 40x lens).
IHC
Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using PTGDS antibody at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IP
IP result of anti-PTGDS (IP: 4ug; Detection:1:600) with mouse brain tissue lysate 1.8mg.| Application Notes | WB:1:500-1:3000 IHC:1:250-1:1000 IP:0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20°C. Stable for one year after shipment. |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
