WB
Western blot analysis of AKR1C3 on different lysates with Rabbit anti-AKR1C3 antibody at 1/1,000 dilution. Lane 1: A549 cell lysate (20 µg/Lane), Lane 2: HepG2 cell lysate (20 µg/Lane), Lane 3: K-562 cell lysate (20 µg/Lane), Lane 4: Human kidney tissue lysate (40 µg/Lane), Lane 5: Mouse kidney tissue lysate (40 µg/Lane), Lane 6: Rat kidney tissue lysate (40 µg/Lane), Exposure time: 20 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.| Product Name | AKR1C3 Recombinant Rabbit Monoclonal Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Recombinant protein within Human AKR1C3 aa 181-280 / 323. |
| Clonality | monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB:1:1000 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Symbol | AKR1C3 |
|---|---|
| Gene Synonyms | DD3 DDX PGFS HAKRB HAKRe HA1753 HSD17B5 hluPGFS |
| Gene Full Name | aldo-keto reductase family 1 member C3 |
| Gene Summary | This gene encodes a member of the aldo/keto reductase superfamily, which consists of more than 40 known enzymes and proteins. These enzymes catalyze the conversion of aldehydes and ketones to their corresponding alcohols by utilizing NADH and/or NADPH as cofactors. The enzymes display overlapping but distinct substrate specificity. This enzyme catalyzes the reduction of prostaglandin (PG) D2, PGH2 and phenanthrenequinone (PQ), and the oxidation of 9alpha,11beta-PGF2 to PGD2. It may play an important role in the pathogenesis of allergic diseases such as asthma, and may also have a role in controlling cell growth and/or differentiation. This gene shares high sequence identity with three other gene members and is clustered with those three genes at chromosome 10p15-p14. Three transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Dec 2011] |
| Alternative Names | 17 beta HSD 5 antibody 17 beta hydroxysteroid dehydrogenase type 5 antibody 17-beta-HSD 5 antibody 17-beta-hydroxysteroid dehydrogenase type 5 antibody 2-dihydrobenzene-1 antibody 2-diol dehydrogenase antibody 20 alpha-hydroxysteroid dehydrogenase antibody 20-alpha-hydroxysteroid dehydrogenase antibody 3 alpha hydroxysteroid dehydrogenase type II antibody 3-alpha-HSD type 2 antibody 17 beta HSD 5 antibody 17 beta hydroxysteroid dehydrogenase type 5 antibody 17-beta-HSD 5 antibody 17-beta-hydroxysteroid dehydrogenase type 5 antibody 2-dihydrobenzene-1 antibody 2-diol dehydrogenase antibody 20 alpha-hydroxysteroid dehydrogenase antibody 20-alpha-hydroxysteroid dehydrogenase antibody 3 alpha hydroxysteroid dehydrogenase type II antibody 3-alpha-HSD type 2 antibody 3-alpha-HSD type II antibody 3-alpha-HSD type II brain antibody 3-alpha-hydroxysteroid dehydrogenase type 2 antibody AK1C3_HUMAN antibody AKR1 C3 antibody Akr1c18 antibody AKR1C3 antibody Aldo keto reductase family 1 member C3 antibody Aldo-keto reductase family 1 member C3 antibody brain antibody Chlordecone reductase antibody Chlordecone reductase homolog HAKRb antibody DD-3 antibody DD3 antibody DDH1 antibody DDX antibody Dihydrodiol dehydrogenase 3 antibody Dihydrodiol dehydrogenase type I antibody Dihydrodiol dehydrogenase X antibody HA1753 antibody HAKRB antibody HAKRe antibody hluPGFS antibody HSD17B5 antibody Indanol dehydrogenase antibody KIAA0119 antibody PGFS antibody Prostaglandin F synthase antibody Testosterone 17-beta-dehydrogenase 5 antibody Trans-1 antibody Trans-1 2-dihydrobenzene-1 2-diol dehydrogenase antibody Type IIb 3 alpha hydroxysteroid dehydrogenase antibody |
| Molecular Weight(MW) | 37kDa(Observed band size: 35kDa) |
| Cellular Localization | Cytoplasm. |
WB
Western blot analysis of AKR1C3 on different lysates with Rabbit anti-AKR1C3 antibody at 1/1,000 dilution. Lane 1: A549 cell lysate (20 µg/Lane), Lane 2: HepG2 cell lysate (20 µg/Lane), Lane 3: K-562 cell lysate (20 µg/Lane), Lane 4: Human kidney tissue lysate (40 µg/Lane), Lane 5: Mouse kidney tissue lysate (40 µg/Lane), Lane 6: Rat kidney tissue lysate (40 µg/Lane), Exposure time: 20 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.| Application Notes | WB:1:1000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
