IHC
Tissue/cell: rat spinal cord; 4% Paraformaldehyde-fixed and paraffin embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum) at 37℃ for 20 min; Incubation: Anti-SCN10A Polyclonal Antibody(1:200), overnight at 4°C, followed by conjugation to the secondary antibodyand DAB staining.IF-P
Tissue/cell: rat brain tissue;4% Paraformaldehyde-fixed and paraffin embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Blocking buffer (normal goat serum) at 37℃ for 20 min; Incubation: Anti-SCN10A Polyclonal Antibody (1:200), overnight at 4°C; The secondary antibody was Goat Anti-Rabbit IgG, Cy3 conjugatedused at 1:200 dilution for 40 minutes at 37°C.| Product Name | SCN10A/Nav1.8 Rabbit pAb |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | KLH conjugated synthetic peptide derived from human SCN10A/NAV1.8 : 1151-1250/1956. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IF-PIHC |
| IHC:1:100-1:500 IF-P:1:100-1:500 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Symbol | SCN10A |
|---|---|
| Gene Synonyms | PN3 SNS FEPS2 Nav1.8 |
| Gene Full Name | sodium voltage-gated channel alpha subunit 10 |
| Gene Summary | The protein encoded by this gene is a tetrodotoxin-resistant voltage-gated sodium channel alpha subunit. The properties of the channel formed by the encoded transmembrane protein can be altered by interaction with different beta subunits. This protein may be involved in the onset of pain associated with peripheral neuropathy. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jun 2014] |
| Alternative Names | FEPS2 Nav1.8 PN3 SNS Na(V)1.8 SCNAA_HUMAN SCN10A Peripheral nerve sodium channel 3 (PN3 | hPN3) Sodium channel protein type X subunit alpha Voltage-gated sodium channel subunit alpha Nav1.8 P70276_MOUSE SCNAA_MOUSE Peripheral nerve sodium channel 3 (PN3) Sensory neuron sodium channel SCNAA_RAT sodium voltage-gated channel alpha subunit 10 sodium channel voltage-gated type X alpha polypeptide sodium channel voltage-gated type X alpha subunit sodium channel voltage gated type X alpha subunit peripheral nerve sodium channel 3 |
| Molecular Weight(MW) | 215kDa |
| Cellular Localization | Membrane; Multi-pass membrane protein. Note=It can be translocated to the extracellular membrane through association with S100A10. |
IHC
Tissue/cell: rat spinal cord; 4% Paraformaldehyde-fixed and paraffin embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum) at 37℃ for 20 min; Incubation: Anti-SCN10A Polyclonal Antibody(1:200), overnight at 4°C, followed by conjugation to the secondary antibodyand DAB staining.
IF-P
Tissue/cell: rat brain tissue;4% Paraformaldehyde-fixed and paraffin embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Blocking buffer (normal goat serum) at 37℃ for 20 min; Incubation: Anti-SCN10A Polyclonal Antibody (1:200), overnight at 4°C; The secondary antibody was Goat Anti-Rabbit IgG, Cy3 conjugatedused at 1:200 dilution for 40 minutes at 37°C.| Application Notes | IHC:1:100-1:500 IF-P:1:100-1:500 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| Storage Buffer | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
