IF-P
Immunofluorescent analysis of (4% PFA) fixed rat brain tissue using Alexa Fluor®555 IBA1 antibody at dilution of 1:200.IF-P
Immunofluorescent analysis of (4% PFA) fixed rat brain tissue using Alexa Fluor®555 IBA1 antibody at dilution of 1:200.FC
1X10^6 THP-1 cells were intracellularly stained with 0.8 ug Alexa Fluor®555 Anti-Human IBA1(red), or 0.8 ug Isotype Control. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer.| Product Name | Alexa Fluor®555-conjugated IBA1 Recombinant antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Recombinant human IBA1 protein. |
| Conjugation | Alexa Fluor® 555 |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | FCIF-P |
| IF-P:1:50-1:500 FC:0.80 ug per 10^6 cells in a 100 µl suspension |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | AIF1 |
|---|---|
| Gene Synonyms | IBA1 IRT1 AIF-1 IRT-1 |
| Gene Full Name | allograft inflammatory factor 1 |
| Gene Summary | This gene encodes a protein that binds actin and calcium. This gene is induced by cytokines and interferon and may promote macrophage activation and growth of vascular smooth muscle cells and T-lymphocytes. Polymorphisms in this gene may be associated with systemic sclerosis. Alternative splicing results in multiple transcript variants, but the full-length and coding nature of some of these variants is not certain. [provided by RefSeq, Jan 2016] |
| Alternative Names | AIF1 4D5 AIF 1 AIF-1 allograft inflammatory factor 1 |
| Molecular Weight(MW) | 17kDa |
| Cellular Localization | Cytoplasm, cytoskeleton,Cell projection, ruffle membrane,Cell projection, phagocytic cup. |
IF-P
Immunofluorescent analysis of (4% PFA) fixed rat brain tissue using Alexa Fluor®555 IBA1 antibody at dilution of 1:200.
IF-P
Immunofluorescent analysis of (4% PFA) fixed rat brain tissue using Alexa Fluor®555 IBA1 antibody at dilution of 1:200.
FC
1X10^6 THP-1 cells were intracellularly stained with 0.8 ug Alexa Fluor®555 Anti-Human IBA1(red), or 0.8 ug Isotype Control. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer.| Application Notes | IF-P:1:50-1:500 FC:0.80 ug per 10^6 cells in a 100 µl suspension |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20°C. Stable for one year after shipment. |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
