ICC/IF
Immunocytochemistry analysis of RAW264.7 cells labeling Iba1 with Rabbit anti-Iba1 antibody at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-Iba1 antibody at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (594) was used as the secondary antibody at 1/1,000 dilution.ICC/IF
Immunocytochemistry analysis of J774A.1 cells labeling Iba1 with Rabbit anti-Iba1 antibody at 1/250 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-Iba1 antibody at 1/250 dilution in 1% BSA in PBST overnight at 4 ℃. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (594) was used as the secondary antibody at 1/1,000 dilution.FC
Flow cytometric analysis of SH-SY5Y cells labeling Iba1. Cells were fixed and permeabilized. Then incubated for 30 minutes at +4℃ with Iba1 (red, 1ug/ml) and Rabbit IgG Isotype Control (488, green, 1ug/ml). Unlabelled sample was used as a control (cells without incubation with primary antibody; black).| Product Name | Alexa Fluor®488 Conjugated Iba1 Recombinant Rabbit Monoclonal Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Synthetic peptide within N-terminal human Iba1. |
| Conjugation | Alexa Fluor® 488 |
| Clonality | monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | FCICC/IF |
| ICC/IF:1:100-1:250 FC:1:500-1:1000 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Symbol | AIF1 |
|---|---|
| Gene Synonyms | IBA1 IRT1 AIF-1 IRT-1 |
| Gene Full Name | allograft inflammatory factor 1 |
| Gene Summary | This gene encodes a protein that binds actin and calcium. This gene is induced by cytokines and interferon and may promote macrophage activation and growth of vascular smooth muscle cells and T-lymphocytes. Polymorphisms in this gene may be associated with systemic sclerosis. Alternative splicing results in multiple transcript variants, but the full-length and coding nature of some of these variants is not certain. [provided by RefSeq, Jan 2016] |
| Alternative Names | AIF 1 antibody AIF-1 antibody Aif1 antibody AIF1 protein antibody AIF1_HUMAN antibody Allograft inflammatory factor 1 antibody Allograft inflammatory factor 1 splice variant G antibody allograft inflammatory factor-1 splice variant Hara-1 antibody balloon angioplasty responsive transcription antibody BART 1 antibody AIF 1 antibody AIF-1 antibody Aif1 antibody AIF1 protein antibody AIF1_HUMAN antibody Allograft inflammatory factor 1 antibody Allograft inflammatory factor 1 splice variant G antibody allograft inflammatory factor-1 splice variant Hara-1 antibody balloon angioplasty responsive transcription antibody BART 1 antibody G1 antibody G1 putative splice variant of allograft inflamatory factor 1 antibody IBA 1 antibody IBA1 antibody interferon gamma responsive transcript antibody Interferon responsive transcript 1 antibody interferon responsive transcript factor 1 antibody Ionized calcium binding adapter molecule 1 antibody Ionized calcium-binding adapter molecule 1 antibody ionized calcium-binding adapter molecule antibody IRT 1 antibody IRT1 antibody Microglia response factor antibody MRF1 antibody Protein g1 antibody |
| Molecular Weight(MW) | 17kDa |
| Cellular Localization | Cytoskeleton, ruffle membrane, phagocytic cup. |
ICC/IF
Immunocytochemistry analysis of RAW264.7 cells labeling Iba1 with Rabbit anti-Iba1 antibody at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-Iba1 antibody at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (594) was used as the secondary antibody at 1/1,000 dilution.
ICC/IF
Immunocytochemistry analysis of J774A.1 cells labeling Iba1 with Rabbit anti-Iba1 antibody at 1/250 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-Iba1 antibody at 1/250 dilution in 1% BSA in PBST overnight at 4 ℃. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (594) was used as the secondary antibody at 1/1,000 dilution.
FC
Flow cytometric analysis of SH-SY5Y cells labeling Iba1. Cells were fixed and permeabilized. Then incubated for 30 minutes at +4℃ with Iba1 (red, 1ug/ml) and Rabbit IgG Isotype Control (488, green, 1ug/ml). Unlabelled sample was used as a control (cells without incubation with primary antibody; black).| Application Notes | ICC/IF:1:100-1:250 FC:1:500-1:1000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
