WB
Various whole cell lysates were separated by 4-20% SDS-PAGE, and the membrane was blotted with anti-PKD1 antibody. The HRP-conjugated Goat anti-Rabbit IgG (H + L) antibody was used to detect the antibody. Lane 1: K562, Lane 2: U14, Lane 3: C6.IHC
Human brain was stained with anti-PKD1 Rabbit antibody.IHC
Rat brain was stained with anti-PKD1 Rabbit antibody.ICC/IF
Immunofluorescence analysis of Hela cell. 1,PKD1 Antibody(green) was diluted at 1:200(4° overnight). 2, Goat Anti Rabbit Alexa Fluor 488 was diluted at 1:1000(room temperature, 50min). 3 DAPI(blue) 10min.| Product Name | PKD1 Rabbit mAb |
|---|---|
| Antibody Type | Primary Antibodies |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHCWB |
| WB:1:2000-1:10000 IHC:1:200-1:1000 ICC/IF:1:200-1:1000 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | PRKD1 |
|---|---|
| Gene Synonyms | PKD PKCM CHDED PRKCM PKC-MU |
| Gene Full Name | protein kinase D1 |
| Gene Summary | The protein encoded by this gene is a serine/threonine protein kinase involved in many cellular processes, including Golgi body membrane integrity and transport, cell migration and differentiation, MAPK8/JNK1 and Ras pathway signaling, MAPK1/3 (ERK1/2) pathway signaling, cell survival, and regulation of cell shape and adhesion. [provided by RefSeq, Jan 2017] |
| Alternative Names | PRKD1 PKD PKD1 PRKCM Serine/threonine-protein kinase D1 Protein kinase C mu type Protein kinase D nPKC-D1 nPKC-mu |
| Molecular Weight(MW) | 102kDa |
| Cellular Localization | Cytoplasm . Cell membrane . Golgi apparatus, trans-Golgi network . Translocation to the cell membrane is required for kinase activation. |
WB
Various whole cell lysates were separated by 4-20% SDS-PAGE, and the membrane was blotted with anti-PKD1 antibody. The HRP-conjugated Goat anti-Rabbit IgG (H + L) antibody was used to detect the antibody. Lane 1: K562, Lane 2: U14, Lane 3: C6.
IHC
Human brain was stained with anti-PKD1 Rabbit antibody.
IHC
Rat brain was stained with anti-PKD1 Rabbit antibody.
ICC/IF
Immunofluorescence analysis of Hela cell. 1,PKD1 Antibody(green) was diluted at 1:200(4° overnight). 2, Goat Anti Rabbit Alexa Fluor 488 was diluted at 1:1000(room temperature, 50min). 3 DAPI(blue) 10min.| Application Notes | WB:1:2000-1:10000 IHC:1:200-1:1000 ICC/IF:1:200-1:1000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | -15°C to -25°C/1 year(Do not lower than -25°C) |
| Storage Buffer | PBS, 50% glycerol, 0.05% Proclin 300, 0.05%BSA |
