WB
Western blot analysis of Phospho-CaMKII (T286) on different lysates with Rabbit anti-Phospho-CaMKII (T286) antibody at 1/1,000 dilution. Lane 1: Human brain tissue lysate, Lane 2: Mouse brain tissue lysate, Lane 3: Rat brain tissue lysate, Lane 4: Mouse brain tissue lysate, the membrane treated with λpp for 1 hour, Lysates/proteins at 30 µg/Lane. Exposure time: 53 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.| Product Name | Phospho-CaMKII (T286) Recombinant Rabbit Monoclonal Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Synthetic phosphopeptide corresponding to residues surrounding Thr286 of human CaMKII. |
| Modification | p-T286 |
| Clonality | monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB:1:1000 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Alternative Names | Calcium/calmodulin dependent protein kinase II alpha antibody Calcium/calmodulin dependent protein kinase II beta antibody Calcium/calmodulin dependent protein kinase II delta antibody Calcium/calmodulin dependent protein kinase II gamma antibody Calcium/calmodulin-dependent protein kinase type II subunit alpha antibody CaM kinase II alpha antibody CaM kinase II antibody CaM kinase II beta antibody CaM kinase II delta antibody CaM kinase II gamma antibody Calcium/calmodulin dependent protein kinase II alpha antibody Calcium/calmodulin dependent protein kinase II beta antibody Calcium/calmodulin dependent protein kinase II delta antibody Calcium/calmodulin dependent protein kinase II gamma antibody Calcium/calmodulin-dependent protein kinase type II subunit alpha antibody CaM kinase II alpha antibody CaM kinase II antibody CaM kinase II beta antibody CaM kinase II delta antibody CaM kinase II gamma antibody CaM kinase II subunit alpha antibody CaMK-II subunit alpha antibody CAMK2 antibody Camk2a antibody CAMK2B antibody CAMK2D antibody CAMK2G antibody CAMKA antibody KCC2A_HUMAN antibody |
|---|---|
| Molecular Weight(MW) | 50-60kDa |
| Function | The Ca2+/calmodulin-dependent protein kinases (CaM kinases) comprise a structurally related subfamily of serine/threonine kinases which include CaMKI, CaMKII and CaMKIV. CaMKII is a ubiquitously expressed serine/ threonine protein kinase that is activated by Ca2+ and calmodulin (CaM) and has been implicated in regulation of the cell cycle and transcription. There are four CaMKII isozymes, designated α, β,γandδ, which may or may not be coexpressed in the same tissue types. CaMKIV is stimulated by Ca2+ and CaM but also requires phosphorylation by a CaMK for full activation. Stimulation of the T cell receptor CD3 signaling complex with an anti-CD3 monoclonal antibody leads to a 10-40 fold increase in CaMKIV activity. An additional kinase, CaMKK, functions to activate CaMKI through the specific phosphorylation of the regulatory threonine residue at position 177. |
| Cellular Localization | Synapse, Postsynaptic density, Cell projection, dendritic spine, dendrite. |
WB
Western blot analysis of Phospho-CaMKII (T286) on different lysates with Rabbit anti-Phospho-CaMKII (T286) antibody at 1/1,000 dilution. Lane 1: Human brain tissue lysate, Lane 2: Mouse brain tissue lysate, Lane 3: Rat brain tissue lysate, Lane 4: Mouse brain tissue lysate, the membrane treated with λpp for 1 hour, Lysates/proteins at 30 µg/Lane. Exposure time: 53 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.| Application Notes | WB:1:1000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
