WB
Various whole cell lysates were separated by 4-20% SDS-PAGE, and the membrane was blotted with anti-Histone H3 (Acetyl Lys27) antibody. The HRP-conjugated Goat anti-Rabbit IgG (H + L) antibody was used to detect the antibody. Lane 1: C6, Lane 2: C6 was treated with TSA(500ng/ml) for 4 hours .IHC
Human brain was stained with anti-Histone H3 (Acetyl Lys27) Rabbit antibody.ICC/IF
Immunofluorescence analysis of Hela cell. 1,Histone H3 (Acetyl Lys27) Monoclona Antibody(red) was diluted at 1:200(4° overnight). CYCS Monoclonal Antibody(green) was diluted at 1:200(4° overnight). 2, Goat Anti Rabbit Alexa Fluor 594 was diluted at 1:1000(room temperature, 50min). Goat Anti Mouse Alexa Fluor 488 was diluted at 1:1000(room temperature, 50min).| Product Name | Histone H3 (Acetyl Lys27) Rabbit mAb |
|---|---|
| Antibody Type | Primary Antibodies |
| Modification | Acetyl Lys27 |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHCWB |
| WB:1:2000-1:10000 IHC:1:200-1:1000 ICC/IF:1:200-1:1000 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | HIST1H3A/HIST1H3B/HIST1H3C/HIST1H3D/HIST1H3E/HIST1H3F/HIST1H3G/HIST1H3H/HIST1H3I/HIST1H3J/HIST2H3A/HIST2H3C/HIST2H3 |
|---|---|
| Gene Summary | Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. This structure consists of approximately 146 bp of DNA wrapped around a nucleosome, an octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a replication-dependent histone that is a member of the histone H3 family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. This gene is found in the large histone gene cluster on chromosome 6p22-p21.3. [provided by RefSeq, Aug 2015] |
| Alternative Names | HIST1H3A H3FA HIST1H3B H3FL HIST1H3C H3FC HIST1H3D H3FB HIST1H3E H3FD HIST1H3F H3FI HIST1H3G H3FH HIST1H3H H3FK HIST1H3I H3FF HIST1H3J H3FJ Histone H3.1 Histone H3/a Histone H3/b Histone H3/c Histone H3/d Histone H3 H3k27AC |
| Molecular Weight(MW) | 15kDa(Observed 17kDa) |
| Cellular Localization | Nucleus. Chromosome. |
WB
Various whole cell lysates were separated by 4-20% SDS-PAGE, and the membrane was blotted with anti-Histone H3 (Acetyl Lys27) antibody. The HRP-conjugated Goat anti-Rabbit IgG (H + L) antibody was used to detect the antibody. Lane 1: C6, Lane 2: C6 was treated with TSA(500ng/ml) for 4 hours .
IHC
Human brain was stained with anti-Histone H3 (Acetyl Lys27) Rabbit antibody.
ICC/IF
Immunofluorescence analysis of Hela cell. 1,Histone H3 (Acetyl Lys27) Monoclona Antibody(red) was diluted at 1:200(4° overnight). CYCS Monoclonal Antibody(green) was diluted at 1:200(4° overnight). 2, Goat Anti Rabbit Alexa Fluor 594 was diluted at 1:1000(room temperature, 50min). Goat Anti Mouse Alexa Fluor 488 was diluted at 1:1000(room temperature, 50min).| Application Notes | WB:1:2000-1:10000 IHC:1:200-1:1000 ICC/IF:1:200-1:1000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | -15°C to -25°C/1 year(Do not lower than -25°C) |
| Storage Buffer | PBS, 50% glycerol, 0.05% Proclin 300, 0.05%BSA |
