WB
Western blot analysis of Nudel on different lysates with Rabbit anti-Nudel antibody at 1/1,000 dilution. Lane 1: Mouse lung tissue lysate, Lane 2: Mouse brain tissue lysate, Lane 3: Rat brain tissue lysate, Lysates/proteins at 30 µg/Lane. Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.IHC-P
Immunohistochemical analysis of paraffin-embedded mouse colon tissue with Rabbit anti-Nudel antibody at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.IHC
Immunohistochemical analysis of paraffin-embedded rat colon tissue with Rabbit anti-Nudel antibody at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.IHC
Immunohistochemical analysis of paraffin-embedded human liver cancer tissue with Rabbit anti-Nudel antibody at 1/50 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/50 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.| Product Name | Nudel Recombinant Rabbit Monoclonal Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Synthetic peptide within Human Nudel aa 1-49 / 345. |
| Clonality | monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IHCWB |
| WB:1:500-1:2000 IHC-P:1:200 |
|
| Species Reactivity | HumanMouseRabbit |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Symbol | NDEL1 |
|---|---|
| Gene Synonyms | EOPA MITAP1 NDE1L1 NDE2 NUDEL |
| Gene Full Name | nudE neurodevelopment protein 1 like 1 |
| Gene Summary | Enables identical protein binding activity. Involved in chromosome segregation; positive regulation of GTPase activity; and regulation of intracellular protein transport. Located in kinetochore. Biomarker of schizophrenia. [provided by Alliance of Genome Resources, Jul 2025] |
| Alternative Names | A. nidulans antibody DKFZp451M0318 antibody ENDOOLIGOPEPTIDASE A antibody EOPA antibody MITAP 1 antibody MITAP1 antibody Mitosin associated protein 1 antibody Mitosin associated protein MITAP1 antibody Mitosin-associated protein 1 antibody Ndel 1 antibody A. nidulans antibody DKFZp451M0318 antibody ENDOOLIGOPEPTIDASE A antibody EOPA antibody MITAP 1 antibody MITAP1 antibody Mitosin associated protein 1 antibody Mitosin associated protein MITAP1 antibody Mitosin-associated protein 1 antibody Ndel 1 antibody NDEL1 antibody NDEL1_HUMAN antibody Nuclear distribution gene E like homolog 1 antibody Nuclear distribution protein nudE like 1 antibody Nuclear distribution protein nudE-like 1 antibody NUDE like protein antibody NudE nuclear distribution gene E homolog like 1 A. nidulans antibody NudE nuclear distribution gene E homolog like 1 antibody NUDEL antibody Protein Nudel antibody |
| Molecular Weight(MW) | 38 kDa(Observed band size: 45 kDa) |
| Cellular Localization | Cytoskeleton. |
WB
Western blot analysis of Nudel on different lysates with Rabbit anti-Nudel antibody at 1/1,000 dilution. Lane 1: Mouse lung tissue lysate, Lane 2: Mouse brain tissue lysate, Lane 3: Rat brain tissue lysate, Lysates/proteins at 30 µg/Lane. Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.
IHC-P
Immunohistochemical analysis of paraffin-embedded mouse colon tissue with Rabbit anti-Nudel antibody at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
IHC
Immunohistochemical analysis of paraffin-embedded rat colon tissue with Rabbit anti-Nudel antibody at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
IHC
Immunohistochemical analysis of paraffin-embedded human liver cancer tissue with Rabbit anti-Nudel antibody at 1/50 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/50 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.| Application Notes | WB:1:500-1:2000 IHC-P:1:200 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
