WB
Various whole cell lysates were separated by 4-20% SDS-PAGE, and the membrane was blotted with anti-Iba1 antibody. The HRP-conjugated Goat anti-Rabbit IgG(H + L) antibody was used to detect the antibody. Lane 1: Mouse, spleen Lane 2: THP-1, Lane 3: Rat testis.IHC
Human tonsil was stained with anti-Iba1 rabbit antibody.IHC
Mouse liver was stained with anti-Iba1 rabbit antibody.IHC
Rat liver was stained with anti-Iba1 rabbit antibody.IF-P
mouse brain was stained with anti-Iba1 Rabbit antibodyIF-P
Rat liver was stained with anti-Iba1 rabbit antibody.| Product Name | Iba1 Rabbit mAb |
|---|---|
| Antibody Type | Primary Antibodies |
| Clonality | monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IF-PIHCWB |
| WB:1:1000-1:5000 IHC:1:2000-1:4000 IF-P:1:200-1:1000 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Symbol | AIF1 |
|---|---|
| Gene Synonyms | IBA1 IRT1 AIF-1 IRT-1 |
| Gene Full Name | allograft inflammatory factor 1 |
| Gene Summary | This gene encodes a protein that binds actin and calcium. This gene is induced by cytokines and interferon and may promote macrophage activation and growth of vascular smooth muscle cells and T-lymphocytes. Polymorphisms in this gene may be associated with systemic sclerosis. Alternative splicing results in multiple transcript variants, but the full-length and coding nature of some of these variants is not certain. [provided by RefSeq, Jan 2016] |
| Alternative Names | AIF 1 AIF-1 Aif1 AIF1 protein AIF1_HUMAN Allograft inflammatory factor 1 Allograft inflammatory factor 1 splice variant G allograft inflammatory factor-1 splice variant Hara-1 balloon angioplasty responsive transcription BART 1 G1 G1 putative splice variant of allograft inflamatory factor 1 IBA 1 IBA1 interferon gamma responsive transcript Interferon responsive transcript 1 interferon responsive transcript factor 1 Ionized calcium binding adapter molecule 1 Ionized calcium-binding adapter molecule 1 ionized calcium-binding adapter molecule IRT 1 IRT1 Microglia response factor MRF1 Protein g1 |
| Molecular Weight(MW) | 17kDa |
| Cellular Localization | Cytoplasm. |
WB
Various whole cell lysates were separated by 4-20% SDS-PAGE, and the membrane was blotted with anti-Iba1 antibody. The HRP-conjugated Goat anti-Rabbit IgG(H + L) antibody was used to detect the antibody. Lane 1: Mouse, spleen Lane 2: THP-1, Lane 3: Rat testis.
IHC
Human tonsil was stained with anti-Iba1 rabbit antibody.
IHC
Mouse liver was stained with anti-Iba1 rabbit antibody.
IHC
Rat liver was stained with anti-Iba1 rabbit antibody.
IF-P
mouse brain was stained with anti-Iba1 Rabbit antibody
IF-P
Rat liver was stained with anti-Iba1 rabbit antibody.| Application Notes | WB:1:1000-1:5000 IHC:1:2000-1:4000 IF-P:1:200-1:1000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | -15°C to -25°C/1 year(Do not lower than -25°C) |
| Storage Buffer | PBS, 50% glycerol, 0.05% Proclin 300, 0.05%BSA. |
