WB
Various lysates were subjected to SDS PAGE followed by western blot with CMAS antibody at dilution of 1:4000 incubated at room temperature for 1.5 hours.IHC
Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using CMAS antibody at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).ICC/IF
Immunofluorescent analysis of (4% PFA) fixed A431 cells using CMAS antibody at dilution of 1:400 and Omnimabs® 488 Goat Anti-Rabbit IgG(H&L).| Product Name | CMAS Polyclonal antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Recombinant human CMAS protein. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHCWB |
| WB:1:1000-1:8000 IHC:1:400-1:1600 ICC/IF:1:200-1:800 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 0.5mg/ml |
| Purification | Affinity purified |
| Gene Symbol | CMAS |
|---|---|
| Gene Synonyms | CSS |
| Gene Full Name | cytidine monophosphate N-acetylneuraminic acid synthetase |
| Gene Summary | This gene encodes an enzyme that converts N-acetylneuraminic acid (NeuNAc) to cytidine 5'-monophosphate N-acetylneuraminic acid (CMP-NeuNAc). This process is important in the formation of sialylated glycoprotein and glycolipids. This modification plays a role in cell-cell communications and immune responses. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Feb 2016] |
| Alternative Names | CMP Neu5Ac SYNTHETASE CMP NeuNAc synthase CMP-N-acetylneuraminic acid synthase CMP-NeuNAc synthase EC:2.7.7.43 |
| Molecular Weight(MW) | 48kDa |
| Cellular Localization | Nucleus. |
WB
Various lysates were subjected to SDS PAGE followed by western blot with CMAS antibody at dilution of 1:4000 incubated at room temperature for 1.5 hours.
IHC
Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using CMAS antibody at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
ICC/IF
Immunofluorescent analysis of (4% PFA) fixed A431 cells using CMAS antibody at dilution of 1:400 and Omnimabs® 488 Goat Anti-Rabbit IgG(H&L).| Application Notes | WB:1:1000-1:8000 IHC:1:400-1:1600 ICC/IF:1:200-1:800 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20°C. Stable for one year after shipment. |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
