WB
Western blot analysis of MAP1B using anti-MAP1B antibody. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human A549 whole cell lysates, Lane 2: human U251 whole cell lysates, Lane 3: human SH-SY5Y whole cell lysates, Lane 4: human U20S whole cell lysates, Lane 5: rat brain tissue lysates, Lane 6: rat C6 whole cell lysates, Lane 7: mouse Neuro-2a whole cell lysates. After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-MAP1B antigen affinity purified polyclonal antibody at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody. The signal is developed using ECL Plus Western Blotting Substrate.IHC
IHC analysis of MAP1B using anti-MAP1B antibody. MAP1B was detected in a paraffin-embedded section of mouse brain tissue. The tissue section was incubated with rabbit anti-MAP1B Antibody at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit with DAB as the chromogen.IHC
IHC analysis of MAP1B using anti-MAP1B antibody. MAP1B was detected in a paraffin-embedded section of rat brain tissue. The tissue section was incubated with rabbit anti-MAP1B Antibody at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit with DAB as the chromogen.IF-P
IF analysis of MAP1B using anti-MAP1B antibody. MAP1B was detected in a paraffin-embedded section of mouse brain tissue. The tissue section was incubated with rabbit anti-MAP1B Antibody at a dilution of 1:100. HRP Conjugated Goat Anti-Rabbit IgG was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. Tyramide signal amplification was performed using TSA 620 reagent at 1:200 dilution at room temperature for 10 minutes. Fluorescence signals were visualized using a fluorescence microscope with filter sets appropriate for TSA 620 and DAPI.IF-P
IF analysis of MAP1B using anti-MAP1B antibody. MAP1B was detected in a paraffin-embedded section of rat brain tissue. The tissue section was incubated with rabbit anti-MAP1B Antibody at a dilution of 1:100. HRP Conjugated Goat Anti-Rabbit IgG was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. Tyramide signal amplification was performed using TSA 620 reagent at 1:200 dilution at room temperature for 10 minutes. Fluorescence signals were visualized using a fluorescence microscope with filter sets appropriate for TSA 620 and DAPI.FC
Flow Cytometry analysis of A549 cells using anti-MAP1B antibody. Overlay histogram showing A549 cells stained with anti-MAP1B antibody (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-MAP1B Antibody at 1:100 dilution for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG was used as secondary antibody at 1:100 dilution for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG at 1:100 dilution used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.| Product Name | Rabbit polyclonal antibody to MAP1B |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | E.coli-derived human MAP1B recombinant protein (Position: H102-R1849). Human MAP1B shares 89.9% and 89.2% amino acid (aa) sequence identity with mouse and rat MAP1B, respectively. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | FCIF-PIHCWB |
| WB:1:500-1:2000 IHC:1:50-1:400 IF-P:1:50-1:400 FC:1:50-1:200 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 0.5mg/ml |
| Purification | Affinity purified |
| Gene Symbol | MAP1B |
|---|---|
| Gene Synonyms | MAP5 PVNH9 DFNA83 FUTSCH PPP1R102 |
| Gene Full Name | microtubule associated protein 1B |
| Gene Summary | This gene encodes a protein that belongs to the microtubule-associated protein family. The proteins of this family are thought to be involved in microtubule assembly, which is an essential step in neurogenesis. The product of this gene is a precursor polypeptide that presumably undergoes proteolytic processing to generate the final MAP1B heavy chain and LC1 light chain. Gene knockout studies of the mouse microtubule-associated protein 1B gene suggested an important role in development and function of the nervous system. [provided by RefSeq, Jul 2008] |
| Alternative Names | Microtubule-associated protein 1B MAP1B heavy chain MAP-1B MAP1 light chain LC1 |
| Molecular Weight(MW) | 271kDa(Observed MW 310kDa) |
| Cellular Localization | Cell projection,Cytoplasm,Cytoskeleton,Microtubule,Synapse. |
WB
Western blot analysis of MAP1B using anti-MAP1B antibody. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human A549 whole cell lysates, Lane 2: human U251 whole cell lysates, Lane 3: human SH-SY5Y whole cell lysates, Lane 4: human U20S whole cell lysates, Lane 5: rat brain tissue lysates, Lane 6: rat C6 whole cell lysates, Lane 7: mouse Neuro-2a whole cell lysates. After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-MAP1B antigen affinity purified polyclonal antibody at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody. The signal is developed using ECL Plus Western Blotting Substrate.
IHC
IHC analysis of MAP1B using anti-MAP1B antibody. MAP1B was detected in a paraffin-embedded section of mouse brain tissue. The tissue section was incubated with rabbit anti-MAP1B Antibody at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit with DAB as the chromogen.
IHC
IHC analysis of MAP1B using anti-MAP1B antibody. MAP1B was detected in a paraffin-embedded section of rat brain tissue. The tissue section was incubated with rabbit anti-MAP1B Antibody at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit with DAB as the chromogen.
IF-P
IF analysis of MAP1B using anti-MAP1B antibody. MAP1B was detected in a paraffin-embedded section of mouse brain tissue. The tissue section was incubated with rabbit anti-MAP1B Antibody at a dilution of 1:100. HRP Conjugated Goat Anti-Rabbit IgG was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. Tyramide signal amplification was performed using TSA 620 reagent at 1:200 dilution at room temperature for 10 minutes. Fluorescence signals were visualized using a fluorescence microscope with filter sets appropriate for TSA 620 and DAPI.
IF-P
IF analysis of MAP1B using anti-MAP1B antibody. MAP1B was detected in a paraffin-embedded section of rat brain tissue. The tissue section was incubated with rabbit anti-MAP1B Antibody at a dilution of 1:100. HRP Conjugated Goat Anti-Rabbit IgG was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. Tyramide signal amplification was performed using TSA 620 reagent at 1:200 dilution at room temperature for 10 minutes. Fluorescence signals were visualized using a fluorescence microscope with filter sets appropriate for TSA 620 and DAPI.
FC
Flow Cytometry analysis of A549 cells using anti-MAP1B antibody. Overlay histogram showing A549 cells stained with anti-MAP1B antibody (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-MAP1B Antibody at 1:100 dilution for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG was used as secondary antibody at 1:100 dilution for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG at 1:100 dilution used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.| Application Notes | WB:1:500-1:2000 IHC:1:50-1:400 IF-P:1:50-1:400 FC:1:50-1:200 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | 12 months from date of receipt, -20℃ as supplied. 6 months 2 to 8℃ after reconstitution. Avoid repeated freezing and thawing. |
| Storage Buffer | 500ug/ml antibody with PBS, 0.02% NaN3, 1 mg/ml BSA and 50% glycerol. |
