WB
Various lysates were subjected to SDS PAGE followed by western blot with PPAR Gamma antibody at dilution of 1:5000 incubated at room temperature for 1.5 hours.WB
Various lysates were subjected to SDS PAGE followed by western blot with PPAR Gamma antibody at dilution of 1:2000 incubated at room temperature for 1.5 hour.IHC
Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using PPAR Gamma antibody at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).IHC
Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using PPAR Gamma antibody at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).IF-P
Immunofluorescent analysis of (4% PFA) fixed rat liver tissue using PPAR Gamma antibody at dilution of 1:200 and Omnimabs® 488 Goat Anti-Rabbit IgG(H&L).FC
1X10^6 HeLa cells were intracellularly stained with 0.4 ug Anti-Human PPAR Gamma and Omnimabs® 488 Goat Anti-Rabbit IgG(H&L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit.IP
IP result of anti-PPAR Gamma (IP:4ug; Detection:1:2000) with HL-60 cells lysate 2450 ug.| Product Name | PPAR Gamma Polyclonal antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Recombinant human PPARG protein. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | FCIF-PIHCIPWB |
| WB:1:1000-1:5000 IHC:1:200-1:800 IF-P:1:50-1:500 FC:0.40 ug per 10^6 cells in a 100 µl suspension IP:0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 0.65mg/ml |
| Purification | Affinity purified |
| Gene Symbol | PPARG |
|---|---|
| Gene Synonyms | GLM1 CIMT1 FPLD3 NR1C3 PPARG1 PPARG2 PPARG5 PPARgamma |
| Gene Full Name | peroxisome proliferator activated receptor gamma |
| Gene Summary | This gene encodes a member of the peroxisome proliferator-activated receptor (PPAR) subfamily of nuclear receptors. PPARs form heterodimers with retinoid X receptors (RXRs) and these heterodimers regulate transcription of various genes. Three subtypes of PPARs are known: PPAR-alpha, PPAR-delta, and PPAR-gamma. The protein encoded by this gene is PPAR-gamma and is a regulator of adipocyte differentiation. Additionally, PPAR-gamma has been implicated in the pathology of numerous diseases including obesity, diabetes, atherosclerosis and cancer. Alternatively spliced transcript variants that encode different isoforms have been described. [provided by RefSeq, Jul 2008] |
| Alternative Names | PPARG NR1C3 Peroxisome proliferator-activated receptor gamma PPAR-gamma PPARγ |
| Molecular Weight(MW) | 58 kDa |
| Cellular Localization | Cytoplasm,Nucleus. |
WB
Various lysates were subjected to SDS PAGE followed by western blot with PPAR Gamma antibody at dilution of 1:5000 incubated at room temperature for 1.5 hours.
WB
Various lysates were subjected to SDS PAGE followed by western blot with PPAR Gamma antibody at dilution of 1:2000 incubated at room temperature for 1.5 hour.
IHC
Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using PPAR Gamma antibody at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC
Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using PPAR Gamma antibody at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IF-P
Immunofluorescent analysis of (4% PFA) fixed rat liver tissue using PPAR Gamma antibody at dilution of 1:200 and Omnimabs® 488 Goat Anti-Rabbit IgG(H&L).
FC
1X10^6 HeLa cells were intracellularly stained with 0.4 ug Anti-Human PPAR Gamma and Omnimabs® 488 Goat Anti-Rabbit IgG(H&L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit.
IP
IP result of anti-PPAR Gamma (IP:4ug; Detection:1:2000) with HL-60 cells lysate 2450 ug.| Application Notes | WB:1:1000-1:5000 IHC:1:200-1:800 IF-P:1:50-1:500 FC:0.40 ug per 10^6 cells in a 100 µl suspension IP:0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20°C. Stable for one year after shipment. |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
