WB
Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-PDE1B monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human) (1:200).IHC
Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-PDE1B mouse monoclonal antibody. Heat-induced epitope retrieval by EDTA solution buffer pH 8.0 at 120°C for 3 min.ICC/IF
Anti-PDE1B mouse monoclonal antibody immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY PDE1B.FC
Flow cytometric Analysis of Jurkat cells, using anti-PDE1B antibody(Red), compared to a nonspecific negative control antibody, (Blue).| Product Name | PDE1B Mouse Monoclonal Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Full length human recombinant protein of human PDE1B(NP_000915) produced in HEK293T cell. |
| Clonality | monoclonal |
|---|---|
| Isotype | IgG2b |
| Host Species | Mouse |
| Tested Applications | FCICC/IFIHCWB |
| WB:1:2000 IHC:1:150 ICC/IF:1:100 FC:1:100 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Protein A/G |
| Gene Symbol | PDE1B |
|---|---|
| Gene Synonyms | PDE1B1 PDES1B HEL-S-79p |
| Gene Full Name | phosphodiesterase 1B |
| Gene Summary | The protein encoded by this gene belongs to the cyclic nucleotide phosphodiesterase (PDE) family, and PDE1 subfamily. Members of the PDE1 family are calmodulin-dependent PDEs that are stimulated by a calcium-calmodulin complex. This PDE has dual-specificity for the second messengers, cAMP and cGMP, with a preference for cGMP as a substrate. cAMP and cGMP function as key regulators of many important physiological processes. Alternatively spliced transcript variants encoding different isoforms have been described for this gene.[provided by RefSeq, Jul 2011] |
| Alternative Names | PDE1B1 PDES1B |
| Molecular Weight(MW) | 62kDa |
| Cellular Localization | Cytoplasm, cytosol. |
WB
Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-PDE1B monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human) (1:200).
IHC
Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-PDE1B mouse monoclonal antibody. Heat-induced epitope retrieval by EDTA solution buffer pH 8.0 at 120°C for 3 min.
ICC/IF
Anti-PDE1B mouse monoclonal antibody immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY PDE1B.
FC
Flow cytometric Analysis of Jurkat cells, using anti-PDE1B antibody(Red), compared to a nonspecific negative control antibody, (Blue).| Application Notes | WB:1:2000 IHC:1:150 ICC/IF:1:100 FC:1:100 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20°C as received. |
| Storage Buffer | PBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide. |
