WB
Positive WB detected in Recombinant protein, All lanes: NP antibody at 3µg/ml, Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution.| Product Name | Rabbit anti-Influenza A virus NP Polyclonal antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Recombinant Influenza A virus Nucleoprotein protein (1-498AA). |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB:1:500-1:5000 |
|
| Species Reactivity | Influenza A |
| Concentration | 1mg/ml |
| Purification | Protein G |
| Gene Symbol | NP |
|---|---|
| Gene Full Name | nucleoprotein |
| Alternative Names | NP antibody Nucleoprotein antibody Nucleocapsid protein antibody Protein N antibody |
| Molecular Weight(MW) | 71kDa |
| Function | Encapsidates the negative strand viral RNA, protecting it from nucleases. The encapsidated genomic RNA is termed the ribonucleoprotein (RNP) and serves as template for transcription and replication. The RNP needs to be localized in the host nucleus to start an infectious cycle, but is too large to diffuse through the nuclear pore complex. NP comprises at least 2 nuclear localization signals that are responsible for the active RNP import into the nucleus through cellular importin alpha/beta pathway. Later in the infection, nclear export of RNPs are mediated through viral proteins NEP interacting with M1 which binds nucleoproteins. It is possible that nucleoprotein binds directly host exportin-1/XPO1 and plays an active role in RNPs nuclear export. M1 interaction with RNP seems to hide nucleoprotein's nuclear localization signals. Soon after a virion infects a new cell, M1 dissociates from the RNP under acidification of the virion driven by M2 protein. Dissociation of M1 from RNP unmasks nucleoprotein's nuclear localization signals, targeting the RNP to the nucleus. |
| Cellular Localization | Virion. Host nucleus. |
WB
Positive WB detected in Recombinant protein, All lanes: NP antibody at 3µg/ml, Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution.| Application Notes | WB:1:500-1:5000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Upon receipt, store at -20°C or -80°C. Avoid repeated freeze. |
| Storage Buffer | Preservative: 0.03% Proclin 300,Constituents: 50% Glycerol, 0.01M PBS, pH 7.4 |
